Tbio TSEN2 tRNA-splicing endonuclease subunit Sen2

Protein Summary

Description

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]

Uniprot Accession IDs

• Q8NCE0
• B7Z6K1
• C9IZI7
• G5E9Q3
• Q8WTW7
• Q9BPU7

Gene Name

• TSEN2

Ensembl ID

• ENST00000284995
• ENSP00000284995
• ENSG00000154743
• ENST00000402228
• ENSP00000385976
• ENST00000415684
• ENSP00000416510
• ENST00000444864
• ENSP00000407974
• ENST00000454502
• ENSP00000392029

Symbol

• SEN2
• SEN2
• PCH2B
• SEN2L

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
histone modification site profile	0.83
transcription factor binding site profile	0.77
small molecule perturbation	0.68
cellular component	0.67
tissue sample	0.66

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Nuclease / Endodeoxyribonuclease / Trna-splicing Endonuclease Subunit Sen2

Protein / Nucleic Acid Binding / Dna Binding Protein / Endodeoxyribonuclease / Trna-splicing Endonuclease Subunit Sen2

Protein / Hydrolase / Trna-splicing Endonuclease Subunit Sen2

DTO Classes

Protein / Enzyme / Nuclease / Endodeoxyribonuclease / Trna-splicing Endonuclease Subunit Sen2

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 32.74   (req: < 5)

Gene RIFs: 1   (req: <= 3)

Antibodies: 182   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 32.74   (req: >= 5)

Gene RIFs: 1   (req: > 3)

Antibodies: 182   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 6

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0