Tbio SUPT4H1 Transcription elongation factor SPT4

Protein Summary

Description

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]

Uniprot Accession IDs

• P63272
• B2R4X8
• D3DTZ4
• Q16550
• Q62387
• Q6ZP89
• hSPT4

Gene Name

• SUPT4H1

Ensembl ID

• ENST00000225504
• ENSP00000225504
• ENSG00000213246
• ENST00000580947
• ENSP00000462670

Symbol

• SPT4H
• SUPT4H
• SPT4
• SPT4H
• SUPT4H
• Supt4a

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
transcription factor binding site profile	0.89
histone modification site profile	0.88
pathway	0.78
biological process	0.76
kinase perturbation	0.76

Protein Classes

PANTHER Classes

Protein / Transcription Factor / Transcription Elongation Factor Spt4

Protein / Nucleic Acid Binding / Dna Binding Protein / Chromatin/chromatin-binding Protein / Transcription Elongation Factor Spt4

DTO Classes

Protein / Nucleic Acid Binding / Dna Binding Protein / Chromatin/chromatin-binding Protein / Transcription Elongation Factor Spt4

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 84.09   (req: < 5)

Gene RIFs: 11   (req: <= 3)

Antibodies: 144   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 84.09   (req: >= 5)

Gene RIFs: 11   (req: > 3)

Antibodies: 144   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 16

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0