Tchem MAP3K20 Mitogen-activated protein kinase kinase kinase 20

Protein Classes

PANTHER Classes

Protein / Transferase / Kinase / Protein Kinase / Mitogen-activated Protein Kinase Kinase Kinase 20

DTO Classes

Protein / Kinase / Protein Kinase / Tkl Group / Mlk Family / Mlk Subfamily / Mitogen-activated Protein Kinase Kinase Kinase Mlt

Protein Summary

Description

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (PubMed:10924358, PubMed:11836244, PubMed:15342622, PubMed:21224381). Involved in limb development (PubMed:26755636). Isoform 1: Phosphorylates histone H3 at 'Ser-28' (PubMed:15684425). May have role in neoplastic cell transformation and cancer development (PubMed:15172994). Causes cell shrinkage and disruption of actin stress fibers (PubMed:11042189). This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• Q9NYL2
• B3KPG2
• Q53SX1
• Q580W8
• Q59GY5
• Q86YW8
• Q9HCC4
• Q9HCC5
• Q9HDD2
• Q9NYE9

Gene Name

• MAP3K20

Ensembl ID

• ENST00000338983
• ENSP00000340257
• ENSG00000091436
• ENST00000375213
• ENSP00000364361
• ENST00000409176
• ENSP00000387259
• ENST00000539448
• ENSP00000439414

Symbol

• MLTK
• ZAK
• pk
• AZK
• MLT
• MRK
• ZAK
• CNM6
• MLK7
• MLTK
• SFMMP
• mlklak
• MLTKbeta
• MLTKalpha

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
biological process	0
biological term	0
cell line	0
cell type or tissue	0
cellular component	0

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 18.74   (req: < 5)

Gene RIFs: 19   (req: <= 3)

Antibodies: 270   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 18.74   (req: >= 5)

Gene RIFs: 19   (req: > 3)

Antibodies: 270   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 20

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 3

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drugs: 2

Approved Drugs (2)

Explore Approved Drugs

1 – 2 of 2

sorafenib

MAP3K20Tchem Kinase

Kd: 8.2

nilotinib

MAP3K20Tchem Kinase

Kd: 7.96

Active Ligands (3)

Explore Active Ligands

1 – 3 of 3

CHEMBL574738

MAP3K20Tchem Kinase

Kd: 8.64

CHEMBL572881

MAP3K20Tchem Kinase

Kd: 8.1

compound 15b [PMID: 16539403]

MAP3K20Tchem Kinase

IC50: 6.1500001

Protein Data Bank (2)

1 – 2 of 2

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (12)

KEGG (1)

PathwayCommons (1)

WikiPathways (10)

Items per page:

5

1 – 1 of 1

Data Source	Name	Explore in Pharos	Explore in Source
KEGG	MAPK signaling pathway	295 targets	path:hsa04010

Name	Explore in Pharos	Explore in Source
MAPK signaling pathway	295 targets	path:hsa04010

Gene Ontology Terms (23)

Functions (5)

Components (3)

Processes (15)

Items per page:

10

1 – 5 of 5

GO Term	Evidence	Assigned by
ATP binding	Inferred from Direct Assay (IDA)	UniProtKB
magnesium ion binding	Inferred from Direct Assay (IDA)	UniProtKB
MAP kinase kinase kinase activity	Inferred from Direct Assay (IDA)	UniProtKB
protein serine/threonine kinase activity	Inferred from Direct Assay (IDA)	UniProtKB
RNA binding	Inferred from High Throughput Direct Assay (HDA)	UniProtKB

Protein-Protein Interactions (20)

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1 – 10 of 20

• See Interactions on String-DB

ZNF544

Tdark

Family: TF

Novelty: 2

p_int: 0.999999953

p_ni: 4.7e-8

Score: 0.642

Data Source: BioPlex,STRINGDB

ZNF227

Tdark

Family: TF

Novelty: 2

p_int: 0.999998116

p_ni: 0.000001883

Score: 0.513

Data Source: BioPlex,STRINGDB

ZNF71

Tdark

Family: TF

Novelty: 0.25641026

p_int: 0.884231373

p_ni: 0.010571824

p_wrong: 0.105196804

Score: 0.234

Data Source: BioPlex,STRINGDB

PKN1

Tchem

Family: Kinase

Novelty: 0.00885136

Score: 0.951

Data Source: STRINGDB

MAP2K6

Tchem

Family: Kinase

Novelty: 0.00880457

Score: 0.947

Data Source: STRINGDB

MAP2K7

Tchem

Family: Kinase

Novelty: 0.0002234

Score: 0.897

Data Source: STRINGDB

ZNF33A

Tdark

Family: TF

Novelty: 0.28659366

Score: 0.823

Data Source: STRINGDB

ZNF567

Tdark

Family: TF

Novelty: 15

Score: 0.703

Data Source: STRINGDB

MAP2K4

Tchem

Family: Kinase

Novelty: 0.00352612

Score: 0.595

Data Source: STRINGDB

SLC25A20

Tbio

Family: Transporter

Novelty: 0.00531784

Score: 0.531

Data Source: STRINGDB

Publication Statistics

PubMed Score  18.74

PubMed score by year

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PubTator Score  229.94

PubTator score by year

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Patents

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Amino Acid Sequence

Residue Counts

Sequence

MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYG

1-70

VILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVV

70-140

IAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLE

140-210

GLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNK

210-280

AEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQ

280-350

LVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPL

350-420

IKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKM

420-490

TKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGN

490-560

PGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPV

560-630

NQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKS

630-700

QHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTK

700-770

VEYRKKPHRPSPAKTNKERARGDHRGWRNF

770-800

MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWRNF

Find similar targets by:

Uniprot Keyword

3D-structure
Acetylation
Alternative splicing
ATP-binding
Cell cycle
Complete proteome
Cytoplasm
Disease mutation
Kinase
Magnesium