Tbio SMURF2 E3 ubiquitin-protein ligase SMURF2

Protein Classes

PANTHER Classes

Protein / Ligase / Ubiquitin-protein Ligase / E3 Ubiquitin-protein Ligase Smurf2

DTO Classes

Protein / Enzyme / Ligase / Ubiquitin-protein Ligase / E3 Ubiquitin-protein Ligase Smurf2

Protein Summary

Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Uniprot Accession IDs

• Q9HAU4
• Q52LL1
• Q9H260
• hSMURF2

Gene Name

• SMURF2

Ensembl ID

• ENST00000262435
• ENSP00000262435
• ENSG00000108854

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
hub protein	0.92
biological process	0.89
interacting protein	0.84
kinase perturbation	0.81
virus perturbation	0.68

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 84.69   (req: < 5)

Gene RIFs: 55   (req: <= 3)

Antibodies: 291   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 84.69   (req: >= 5)

Gene RIFs: 55   (req: > 3)

Antibodies: 291   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 21

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Data Bank (6)

1 – 5 of 6

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (126)

Reactome (29)

KEGG (4)

PathwayCommons (8)

UniProt (1)

WikiPathways (84)

Adaptive Immune System (R-HSA-1280218)

Click on a row in the table to change the structure displayed.

Items per page:

5

1 – 5 of 29

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Adaptive Immune System	894 targets	R-HSA-1280218
Reactome	Antigen processing: Ubiquitination & Proteasome degradation	307 targets	R-HSA-983168
Reactome	Asymmetric localization of PCP proteins	65 targets	R-HSA-4608870
Reactome	Beta-catenin independent WNT signaling	146 targets	R-HSA-3858494
Reactome	Class I MHC mediated antigen processing & presentation	437 targets	R-HSA-983169

Name	Explore in Pharos	Explore in Source
Adaptive Immune System	894 targets	R-HSA-1280218
Antigen processing: Ubiquitination & Proteasome degradation	307 targets	R-HSA-983168
Asymmetric localization of PCP proteins	65 targets	R-HSA-4608870
Beta-catenin independent WNT signaling	146 targets	R-HSA-3858494
Class I MHC mediated antigen processing & presentation	437 targets	R-HSA-983169

Gene Ontology Terms (29)

Functions (5)

Components (8)

Processes (16)

Items per page:

10

1 – 5 of 5

GO Term	Evidence	Assigned by
ubiquitin-protein transferase activity	Inferred from Direct Assay (IDA)	UniProtKB
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
SMAD binding	Inferred from Physical Interaction (IPI)	BHF-UCL
ubiquitin protein ligase activity	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
transforming growth factor beta receptor binding	Inferred from Electronic Annotation (IEA)	Ensembl

Protein-Protein Interactions (428)

Explore Interacting Targets

1 – 10 of 428

• See Interactions on String-DB

SMURF1

Tchem

Family: Enzyme

Novelty: 0.00980819

p_int: 1

Score: 0.983

Data Source: BioPlex,STRINGDB

SMAD5

Tbio

Family: TF

Novelty: 0.00607935

p_int: 0.999999913

p_ni: 5.8e-8

p_wrong: 3e-8

Score: 0.982

Data Source: BioPlex,STRINGDB

SMAD1

Tbio

Family: TF

Novelty: 0.0421653

p_int: 0.999999089

p_ni: 9.1e-7

Score: 0.984

Data Source: BioPlex,STRINGDB

SEMG2

Tbio

Novelty: 0.10545717

p_int: 0.999998452

p_ni: 0.000001548

Score: 0.183

Data Source: BioPlex,STRINGDB

MARS2

Tbio

Family: Enzyme

Novelty: 0.03610718

p_int: 0.999989794

p_ni: 0.000010205

p_wrong: 1e-9

Score: 0.201

Data Source: BioPlex,STRINGDB

ARHGAP5

Tbio

Family: Enzyme

Novelty: 0.0530051

p_int: 0.999972752

p_ni: 0.000027248

Score: 0.361

Data Source: BioPlex,STRINGDB

LINGO2

Tbio

Novelty: 0.08773809

p_int: 0.999815954

p_ni: 0.000184046

Score: 0.19

Data Source: BioPlex,STRINGDB

LDLRAD4

Tbio

Novelty: 0.02672907

p_int: 0.998611343

p_ni: 0.001312434

p_wrong: 0.000076223

Score: 0.614

Data Source: BioPlex,STRINGDB

NEDD4

Tchem

Family: Enzyme

Novelty: 0.00341025

p_int: 0.99038598

p_ni: 0.00961402

p_wrong: 1e-9

Score: 0.957

Data Source: BioPlex,STRINGDB

CUEDC1

Tdark

Novelty: 0.26785714

p_int: 0.974652491

p_ni: 0.006209181

p_wrong: 0.019138328

Data Source: BioPlex

Publication Statistics

PubMed Score  84.69

PubMed score by year

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PubTator Score  65.12

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIG

1-70

KSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD

70-140

RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENT

140-210

PISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWH

210-280

DPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQ

280-350

QVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWK

350-420

RLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIM

420-490

GMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEII

490-560

QHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLG

560-630

KIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTI

630-700

HQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

700-748

MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Find similar targets by:

Uniprot Keyword

3D-structure
Cell membrane
Complete proteome
Cytoplasm
Membrane
Nucleus
Reference proteome
Repeat
Transferase
Ubl conjugation