Tbio RFK Riboflavin kinase

Protein Classes

PANTHER Classes

Protein / Transferase / Kinase / Riboflavin Kinase

DTO Classes

Protein / Enzyme / Transferase / Riboflavin Kinase

Protein Summary

Description

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]

Uniprot Accession IDs

• Q969G6
• Q5JSG9
• Q9NUT7

Gene Name

• RFK

Ensembl ID

• ENST00000376736
• ENSP00000365926
• ENSG00000135002

Symbol

• RIFK

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
kinase perturbation	1
disease perturbation	0.93
molecular function	0.91
histone modification site profile	0.86
transcription factor perturbation	0.8

Related Tools

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IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 105   (req: < 5)

Gene RIFs: 6   (req: <= 3)

Antibodies: 144   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 105   (req: >= 5)

Gene RIFs: 6   (req: > 3)

Antibodies: 144   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 9

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Orthologs (12)

1 – 5 of 12

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	riboflavin kinase	VGNC:13953	744462
Mouse	riboflavin kinase	MGI:1914688	54391
Rat	riboflavin kinase	RGD:1549748	499328
Cow	riboflavin kinase	VGNC:33891	514697
Opossum	riboflavin kinase		100019592

Species	Name	OMA	EggNOG	Inparanoid
Chimp	riboflavin kinase
Mouse	riboflavin kinase
Rat	riboflavin kinase
Cow	riboflavin kinase
Opossum	riboflavin kinase

Protein Structure (4 Structures, 1 AlphaFold Model)

tutorial

Representation

  axes   backbone   ball+stick   cartoon   rocket   rope   spacefill

Color Scheme

  target   atomindex   bfactor   chainname   electrostatic   element   hydrophobicity   resname   sstruc

Click on a row in the table to change the structure displayed.
More information can be found at RCSB PDB

AF-Q969G6-F1-model_v1

AlphaFold Structures Developed by DeepMind and EMBL-EBI

1 – 4 of 4

PDB Structure Id	Ligand	Method	Resolution (Å)	Residues	Fraction of Total Protein	Pub Year	Title

PDB Structure Id	Fraction of Total Protein	Resolution	Pub Year

Pathways (13)

Reactome (4)

KEGG (2)

PathwayCommons (3)

UniProt (1)

WikiPathways (3)

Metabolism (R-HSA-1430728)

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Find Similar Targets

Items per page:

5

1 – 4 of 4

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Metabolism	2112 targets	R-HSA-1430728
Reactome	Metabolism of vitamins and cofactors	188 targets	R-HSA-196854
Reactome	Metabolism of water-soluble vitamins and cofactors	122 targets	R-HSA-196849
Reactome	Vitamin B2 (riboflavin) metabolism	7 targets	R-HSA-196843

Name	Explore in Pharos	Explore in Source
Metabolism	2112 targets	R-HSA-1430728
Metabolism of vitamins and cofactors	188 targets	R-HSA-196854
Metabolism of water-soluble vitamins and cofactors	122 targets	R-HSA-196849
Vitamin B2 (riboflavin) metabolism	7 targets	R-HSA-196843

Gene Ontology Terms (11)

Functions (3)

Components (2)

Processes (6)

Find Similar Targets

Items per page:

10

1 – 3 of 3

GO Term	Evidence	Assigned by
riboflavin kinase activity	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
ATP binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW
metal ion binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW

Protein-Protein Interactions (77)

Explore Interacting Targets

1 – 10 of 77

• See Interactions on String-DB

FLAD1

Tbio

Family:  Enzyme

Novelty:  0.02230368

Score:  0.996

Data Source:  STRINGDB

ENPP3

Tchem

Family:  Enzyme

Novelty:  0.00414129

Score:  0.918

Data Source:  STRINGDB

ENPP1

Tchem

Family:  Enzyme

Novelty:  0.00256132

Score:  0.916

Data Source:  STRINGDB

BLVRB

Tbio

Family:  Enzyme

Novelty:  0.00705559

Score:  0.9

Data Source:  STRINGDB

YKT6

Tbio

Novelty:  0.01591063

Score:  0.852

Data Source:  STRINGDB

ACP5

Tbio

Family:  Enzyme

Novelty:  0.00034703

Score:  0.831

Data Source:  STRINGDB

UFM1

Tbio

Novelty:  0.0215914

Score:  0.825

Data Source:  STRINGDB

ACP2

Tbio

Family:  Enzyme

Novelty:  0.00355652

Score:  0.823

Data Source:  STRINGDB

TNFRSF1A

Tchem

Novelty:  0.00037847

Score:  0.823

Data Source:  STRINGDB

MEMO1

Tbio

Novelty:  0.0506558

Score:  0.807

Data Source:  STRINGDB

Publication Statistics

PubMed Score  105.00

PubMed score by year

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PubTator Score  19.02

PubTator score by year

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Amino Acid Sequence

Residue Counts

Protein Sequence

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UniProt Keyword Find Similar Targets

No Header	No Header
3D-structure
ATP-binding
Complete proteome
Cytoplasm
Flavoprotein
FMN
Kinase
Magnesium
Metal-binding
Nucleotide-binding

MGI Phenotype Find Similar Targets

No Header	No Header
hematopoietic system phenotype
homeostasis/metabolism phenotype
immune system phenotype
mortality/aging