Tchem NEK9 Serine/threonine-protein kinase Nek9

Protein Classes

DTO Classes

Protein / Kinase / Protein Kinase / Other Group / Nek Family / Serine/threonine-protein Kinase Nek9

Protein Summary

Description

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016]

Uniprot Accession IDs

• Q8TD19
• Q52LK6
• Q8NCN0
• Q8TCY4
• Q9UPI4
• Q9Y6S4
• Q9Y6S5
• Q9Y6S6

Gene Name

• NEK9

Ensembl ID

• ENST00000238616
• ENSP00000238616
• ENSG00000119638

Symbol

• KIAA1995
• NEK8
• NERCC
• NC
• APUG
• NERCC
• LCCS10
• NERCC1

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
interacting protein	1
virus perturbation	0.98
drug perturbation	0.96
molecular function	0.96
histone modification site profile	0.88

Related Tools

Dark Kinase Knowledgebase

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ProKinO

The ProKinO is a protein kinase-specific ontology, which provides a controlled vocabulary of terms, their hierarchy, and relationships unifying sequence, structure, function, mutation and pathway information on kinases.

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Target Illumination GWAS Analytics (TIGA)

TIGA scores and ranks GWAS discovered associations according to the quantity and quality of the evidence supporting the association.

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GENEVA

GENEVA (GENe Expression Variance Analysis) allows you to identify RNA-sequencing datasets from the Gene Expression Omnibus (GEO) that contain conditions modulating a gene or a gene signature.

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GlyGen

GlyGen is a data integration and dissemination project for carbohydrate and glycoconjugate related data.

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ARCHS4

ARCHS4 provides access to gene-function predictions based on RNA-seq co-expression, and gene expression levels across cell and tissues.

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IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 46.5   (req: < 5)

Gene RIFs: 20   (req: <= 3)

Antibodies: 446   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 46.5   (req: >= 5)

Gene RIFs: 20   (req: > 3)

Antibodies: 446   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 6

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 1

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

IDG Resources

Data Resources (1)

NanoBRET

NanoLuc®-NEK9

• Institute: UNC-CH
• Authors: Julie Pickett
• PI: Tim Willson
• Assay ID: BAO_0010069
• Endpoint: fold change
• Endpoint Detection: fluorescence intensity
• Data: NanoBRET Assay Tracer Titration
• Repository: Synapse

Active Ligands (1)

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CHEMBL475251

NEK9Tchem Kinase

Kd : 7.8

GWAS Traits (1)

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GWAS Trait	EFO ID	Study Count	SNP Count	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
coronary artery disease	EFO_0001645	2	1	2		34.7

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GWAS Trait	EFO ID	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
coronary artery disease	EFO_0001645	2		34.7

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Orthologs (12)

1 – 5 of 12

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	NIMA related kinase 9	VGNC:7449	467512
Macaque	NIMA related kinase 9		701707
Mouse	NIMA (never in mitosis gene a)-related expressed kinase 9	MGI:2387995	217718
Rat	NIMA-related kinase 9	RGD:1307187	299204
Dog	NIMA related kinase 9	VGNC:43735	490790

Species	Name	OMA	EggNOG	Inparanoid
Chimp	NIMA related kinase 9
Macaque	NIMA related kinase 9
Mouse	NIMA (never in mitosis gene a)-related expressed kinase 9
Rat	NIMA-related kinase 9
Dog	NIMA related kinase 9

Protein Structure (2 Structures, 1 AlphaFold Model)

Representation

  axes   backbone   ball+stick   cartoon   rocket   rope   spacefill

Color Scheme

  target   atomindex   bfactor   chainname   electrostatic   element   hydrophobicity   resname   sstruc

Click on a row in the table to change the structure displayed.
More information can be found at RCSB PDB

AF-Q8TD19-F1-model_v1

AlphaFold Structures Developed by DeepMind and EMBL-EBI

1 – 2 of 2

PDB Structure Id	Ligand	Method	Resolution (Å)	Residues	Fraction of Total Protein	Pub Year	Title

PDB Structure Id	Fraction of Total Protein	Resolution	Pub Year

Pathways (7)

Reactome (7)

Activation of NIMA Kinases NEK9, NEK6, NEK7 (R-HSA-2980767)

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Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Activation of NIMA Kinases NEK9, NEK6, NEK7	7 targets	R-HSA-2980767
Reactome	Cell Cycle	597 targets	R-HSA-1640170
Reactome	Cell Cycle, Mitotic	495 targets	R-HSA-69278
Reactome	M Phase	350 targets	R-HSA-68886
Reactome	Mitotic Prophase	113 targets	R-HSA-68875

Name	Explore in Pharos	Explore in Source
Activation of NIMA Kinases NEK9, NEK6, NEK7	7 targets	R-HSA-2980767
Cell Cycle	597 targets	R-HSA-1640170
Cell Cycle, Mitotic	495 targets	R-HSA-69278
M Phase	350 targets	R-HSA-68886
Mitotic Prophase	113 targets	R-HSA-68875

Gene Ontology Terms (8)

Functions (4)

Components (2)

Processes (2)

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GO Term	Evidence	Assigned by
protein kinase binding	Inferred from Physical Interaction (IPI)	UniProtKB
ATP binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW
metal ion binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW
protein serine/threonine kinase activity	Inferred from Electronic Annotation (IEA)	UniProtKB-KW

Protein-Protein Interactions (103)

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1 – 10 of 103

• See Interactions on String-DB

NEK6

Tchem

Family: Kinase

Novelty: 0.02394134

p_int: 0.982898868

p_ni: 0.017101132

Score: 0.986

Data Source: BioPlex,Reactome,STRINGDB

MAFF

Tbio

Family: TF

Novelty: 0.01974936

p_int: 0.866894065

p_ni: 0.133105932

p_wrong: 3e-9

Score: 0.234

Data Source: BioPlex,STRINGDB

RNF19B

Tbio

Family: Enzyme

Novelty: 0.20054824

p_int: 0.857389633

p_ni: 0.142610367

Score: 0.202

Data Source: BioPlex,STRINGDB

ITIH5

Tbio

Novelty: 0.07069373

p_int: 0.760441637

p_ni: 0.239558363

Data Source: BioPlex

NEK7

Tchem

Family: Kinase

Novelty: 0.02675561

Score: 0.997

Data Source: STRINGDB

PLK1

Tchem

Family: Kinase

Novelty: 0.00062799

Score: 0.96

Data Source: Reactome,STRINGDB

BICD2

Tbio

Novelty: 0.01725046

Score: 0.946

Data Source: STRINGDB

CDK1

Tchem

Family: Kinase

Novelty: 0.00040776

Score: 0.925

Data Source: Reactome,STRINGDB

NUP93

Tbio

Novelty: 0.0248311

Score: 0.924

Data Source: STRINGDB

CCNB1

Tchem

Novelty: 0.0005503

Score: 0.92

Data Source: Reactome,STRINGDB

Publication Statistics

PubMed Score  46.50

PubMed score by year

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PubTator Score  15.15

PubTator score by year

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Patents

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Amino Acid Sequence

Residue Counts

Protein Sequence

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Ortholog Sequence Conservation

WebLogo Color Code

PolarG S T Y C

NeutralQ N

BasicK R H

AcidicD E

HydrophobicA V L I P W F M

Compare across kinases on ProKinO

Structural Annotations

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UniProt Keyword Find Similar Targets

3D-structure
Acetylation
ATP-binding
Cell cycle
Cell division
Coiled coil
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation

GWAS Trait Find Similar Targets

coronary artery disease

MGI Phenotype Find Similar Targets

hematopoietic system phenotype
homeostasis/metabolism phenotype
immune system phenotype
mortality/aging