Tchem NOTUM Palmitoleoyl-protein carboxylesterase NOTUM

Protein Summary

Description

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).

Uniprot Accession IDs

• Q6P988
• Q8N410
• Q8NI82

Gene Name

• NOTUM

Ensembl ID

• ENST00000409678
• ENSP00000387310
• ENSG00000185269

Symbol

• hNOTUM

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
cell type or tissue	0.78
kinase perturbation	0.76
transcription factor binding site profile	0.74
PubMedID	0.6
transcription factor	0.59

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 165.52   (req: < 5)

Gene RIFs: 4   (req: <= 3)

Antibodies: 156   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 165.52   (req: >= 5)

Gene RIFs: 4   (req: > 3)

Antibodies: 156   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 8

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 63

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Active Ligands (63)

Explore Active Ligands

1 – 10 of 63

CHEMBL3774802

NOTUMTchem Enzyme

EC50: 6.05

CHEMBL3774730

NOTUMTchem Enzyme

EC50: 6.05

CHEMBL3775000

NOTUMTchem Enzyme

EC50: 6.07

CHEMBL3775731

NOTUMTchem Enzyme

EC50: 6.08

CHEMBL3774810

NOTUMTchem Enzyme

EC50: 6.1

CHEMBL3759300

NOTUMTchem Enzyme

EC50: 6.18

CHEMBL1689356

NOTUMTchem Enzyme

EC50: 6.24

CHEMBL3775635

NOTUMTchem Enzyme

EC50: 6.25

CHEMBL3775859

NOTUMTchem Enzyme

EC50: 6.32

CHEMBL3759068

NOTUMTchem Enzyme

EC50: 6.34

Protein Data Bank (12)

1 – 5 of 12

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (75)

Reactome (8)

KEGG (1)

WikiPathways (66)

Hedgehog ligand biogenesis (R-HSA-5358346)

Click on a row in the table to change the structure displayed.

Items per page:

5

1 – 5 of 8

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Hedgehog ligand biogenesis	66 targets	R-HSA-5358346
Reactome	Metabolism of proteins	2050 targets	R-HSA-392499
Reactome	Post-translational protein modification	1450 targets	R-HSA-597592
Reactome	Post-translational protein phosphorylation	107 targets	R-HSA-8957275
Reactome	Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)	124 targets	R-HSA-381426

Name	Explore in Pharos	Explore in Source
Hedgehog ligand biogenesis	66 targets	R-HSA-5358346
Metabolism of proteins	2050 targets	R-HSA-392499
Post-translational protein modification	1450 targets	R-HSA-597592
Post-translational protein phosphorylation	107 targets	R-HSA-8957275
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)	124 targets	R-HSA-381426

Gene Ontology Terms (10)

Functions (2)

Components (2)

Processes (6)

Items per page:

10

1 – 2 of 2

GO Term	Evidence	Assigned by
palmitoleyl hydrolase activity	Inferred from Direct Assay (IDA)	UniProtKB
phospholipase C activity	Traceable Author Statement (TAS)	Reactome

Protein-Protein Interactions (167)

Explore Interacting Targets

1 – 10 of 167

• See Interactions on String-DB

GPC3

Tbio

Novelty: 0.00107694

Score: 0.976

Data Source: STRINGDB

WNT1

Tbio

Novelty: 0.0015436

Score: 0.966

Data Source: STRINGDB

GPC5

Tbio

Novelty: 0.01709985

Score: 0.962

Data Source: Reactome,STRINGDB

SHH

Tchem

Novelty: 0.00033539

Score: 0.938

Data Source: Reactome,STRINGDB

BMP4

Tchem

Novelty: 0.00057391

Score: 0.937

Data Source: STRINGDB

AHSG

Tbio

Novelty: 0.00097441

Score: 0.932

Data Source: STRINGDB

ALB

Tchem

Novelty: 0.0000092

Score: 0.932

Data Source: STRINGDB

IHH

Tbio

Novelty: 0.00230273

Score: 0.928

Data Source: STRINGDB

AFP

Tbio

Novelty: 0.00006259

Score: 0.928

Data Source: STRINGDB

DHH

Tbio

Novelty: 0.00436048

Score: 0.927

Data Source: STRINGDB

Publication Statistics

PubMed Score  165.52

PubMed score by year

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PubTator Score  8.58

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MGRGVRVLLLLSLLHCAGGSEGRKTWRRRGQQPPPPPRTEAAPAAGQPVESFPLDFTAVEGNMDSFMAQV

1-70

KSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESRGSRRWLLFLEGGWYCFNRENCDSRY

70-140

DTMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQE

140-210

VVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCV

210-280

DTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVH

280-350

LTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHD

350-420

SHKASKTPLKGCPVHLVDSCPWPHCNPSCPTVRDQFTGQEMNVAQFLMHMGFDMQTVAQPQGLEPSELLG

420-490

MLSNGS

490-496

MGRGVRVLLLLSLLHCAGGSEGRKTWRRRGQQPPPPPRTEAAPAAGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCNDGSPAGYYLKESRGSRRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRTGTGILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGRGLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRHTDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEIIIRSHWTDVQVKGTSLPRALHCWDRSLHDSHKASKTPLKGCPVHLVDSCPWPHCNPSCPTVRDQFTGQEMNVAQFLMHMGFDMQTVAQPQGLEPSELLGMLSNGS

Find similar targets by:

Uniprot Keyword

3D-structure
Complete proteome
Glycoprotein
Hydrolase
Phosphoprotein
Reference proteome
Secreted
Serine esterase
Signal
Wnt signaling pathway