Tbio IFI16 Gamma-interferon-inducible protein 16

Protein Classes

PANTHER Classes

Protein / Transcription Factor / Gamma-interferon-inducible Protein 16

DTO Classes

Protein / Transcription Factor / Gamma-interferon-inducible Protein 16

Protein Summary

Description

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

Uniprot Accession IDs

• Q16666
• B4DJT8
• H3BLV7
• Q59GX0
• Q5T3W7
• Q5T3W8
• Q5T3X0
• Q5T3X1
• Q5T3X2
• Q8N9E5
• Q8NEQ7
• Q96AJ5
• Q9UH78
• Ifi-16

Gene Name

• IFI16

Ensembl ID

• ENST00000295809
• ENSP00000295809
• ENSG00000163565
• ENST00000359709
• ENSP00000352740
• ENST00000368131
• ENSP00000357113
• ENST00000368132
• ENSP00000357114
• ENST00000448393
• ENSP00000404325

Symbol

• IFNGIP1
• PYHIN2
• IFNGIP1

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
transcription factor perturbation	1
virus perturbation	1
biological process	0.99
kinase perturbation	0.99
disease perturbation	0.97

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 129.8   (req: < 5)

Gene RIFs: 106   (req: <= 3)

Antibodies: 306   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 129.8   (req: >= 5)

Gene RIFs: 106   (req: > 3)

Antibodies: 306   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 32

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Data Bank (6)

1 – 5 of 6

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (91)

Reactome (5)

KEGG (1)

PathwayCommons (1)

WikiPathways (84)

Cytosolic sensors of pathogen-associated DNA (R-HSA-1834949)

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Items per page:

5

1 – 5 of 5

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Cytosolic sensors of pathogen-associated DNA	64 targets	R-HSA-1834949
Reactome	IRF3-mediated induction of type I IFN	13 targets	R-HSA-3270619
Reactome	Immune System	2246 targets	R-HSA-168256
Reactome	Innate Immune System	1188 targets	R-HSA-168249
Reactome	STING mediated induction of host immune responses	16 targets	R-HSA-1834941

Name	Explore in Pharos	Explore in Source
Cytosolic sensors of pathogen-associated DNA	64 targets	R-HSA-1834949
IRF3-mediated induction of type I IFN	13 targets	R-HSA-3270619
Immune System	2246 targets	R-HSA-168256
Innate Immune System	1188 targets	R-HSA-168249
STING mediated induction of host immune responses	16 targets	R-HSA-1834941

Gene Ontology Terms (38)

Functions (6)

Components (6)

Processes (26)

Items per page:

10

1 – 6 of 6

GO Term	Evidence	Assigned by
double-stranded DNA binding	Inferred from Direct Assay (IDA)	UniProtKB
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific	Inferred from Direct Assay (IDA)	NTNU_SB
RNA polymerase II proximal promoter sequence-specific DNA binding	Inferred from Direct Assay (IDA)	NTNU_SB
transcription factor binding	Inferred from Direct Assay (IDA)	UniProtKB
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
RNA binding	Inferred from High Throughput Direct Assay (HDA)	UniProtKB

Protein-Protein Interactions (87)

Explore Interacting Targets

1 – 10 of 87

• See Interactions on String-DB

TMEM173

Tchem

Novelty: 0.00361815

Score: 0.978

Data Source: STRINGDB

IRF3

Tbio

Family: TF

Novelty: 0.00132895

Score: 0.966

Data Source: STRINGDB

TBK1

Tchem

Family: Kinase

Novelty: 0.00150228

Score: 0.965

Data Source: STRINGDB

DDX41

Tbio

Family: Enzyme

Novelty: 0.00021331

Score: 0.959

Data Source: STRINGDB

PYCARD

Tbio

Novelty: 0.00561282

Score: 0.944

Data Source: STRINGDB

CGAS

Tbio

Family: Enzyme

Novelty: 0.00176488

Score: 0.89

Data Source: STRINGDB

AIM2

Tbio

Novelty: 0.00325294

Score: 0.842

Data Source: STRINGDB

CASP1

Tchem

Family: Enzyme

Novelty: 0.00043112

Score: 0.838

Data Source: STRINGDB

DDX58

Tbio

Family: Enzyme

Novelty: 0.00108351

Score: 0.833

Data Source: STRINGDB

TP53

Tchem

Family: TF

Novelty: 0.00002203

Score: 0.779

Data Source: STRINGDB

Publication Statistics

PubMed Score  129.80

PubMed score by year

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PubTator Score  131.5

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MGKKYKNIVLLKGLEVINDYHFRMVKSLLSNDLKLNLKMREEYDKIQIADLMEEKFRGDAGLGKLIKIFE

1-70

DIPTLEDLAETLKKEKLKVKGPALSRKRKKEVDATSPAPSTSSTVKTEGAEATPGAQKRKKSTKEKAGPK

70-140

GSKVSEEQTQPPSPAGAGMSTAMGRSPSPKTSLSAPPNSSSTENPKTVAKCQVTPRRNVLQKRPVIVKVL

140-210

STTKPFEYETPEMEKKIMFHATVATQTQFFHVKVLNTSLKEKFNGKKIIIISDYLEYDSLLEVNEESTVS

210-280

EAGPNQTFEVPNKIINRAKETLKIDILHKQASGNIVYGVFMLHKKTVNQKTTIYEIQDDRGKMDVVGTGQ

280-350

CHNIPCEEGDKLQLFCFRLRKKNQMSKLISEMHSFIQIKKKTNPRNNDPKSMKLPQEQRQLPYPSEASTT

350-420

FPESHLRTPQMPPTTPSSSFFTKKSEDTISKMNDFMRMQILKEGSHFPGPFMTSIGPAESHPHTPQMPPS

420-490

TPSSSFLTTKSEDTISKMNDFMRMQILKEGSHFPGPFMTSIGPAESHPHTPQMPPSTPSSSFLTTLKPRL

490-560

KTEPEEVSIEDSAQSDLKEVMVLNATESFVYEPKEQKKMFHATVATENEVFRVKVFNIDLKEKFTPKKII

560-630

AIANYVCRNGFLEVYPFTLVADVNADRNMEIPKGLIRSASVTPKINQLCSQTKGSFVNGVFEVHKKNVRG

630-700

EFTYYEIQDNTGKMEVVVHGRLTTINCEEGDKLKLTCFELAPKSGNTGELRSVIHSHIKVIKTRKNKKDI

700-770

LNPDSSMETSPDFFF

770-785

MGKKYKNIVLLKGLEVINDYHFRMVKSLLSNDLKLNLKMREEYDKIQIADLMEEKFRGDAGLGKLIKIFEDIPTLEDLAETLKKEKLKVKGPALSRKRKKEVDATSPAPSTSSTVKTEGAEATPGAQKRKKSTKEKAGPKGSKVSEEQTQPPSPAGAGMSTAMGRSPSPKTSLSAPPNSSSTENPKTVAKCQVTPRRNVLQKRPVIVKVLSTTKPFEYETPEMEKKIMFHATVATQTQFFHVKVLNTSLKEKFNGKKIIIISDYLEYDSLLEVNEESTVSEAGPNQTFEVPNKIINRAKETLKIDILHKQASGNIVYGVFMLHKKTVNQKTTIYEIQDDRGKMDVVGTGQCHNIPCEEGDKLQLFCFRLRKKNQMSKLISEMHSFIQIKKKTNPRNNDPKSMKLPQEQRQLPYPSEASTTFPESHLRTPQMPPTTPSSSFFTKKSEDTISKMNDFMRMQILKEGSHFPGPFMTSIGPAESHPHTPQMPPSTPSSSFLTTKSEDTISKMNDFMRMQILKEGSHFPGPFMTSIGPAESHPHTPQMPPSTPSSSFLTTLKPRLKTEPEEVSIEDSAQSDLKEVMVLNATESFVYEPKEQKKMFHATVATENEVFRVKVFNIDLKEKFTPKKIIAIANYVCRNGFLEVYPFTLVADVNADRNMEIPKGLIRSASVTPKINQLCSQTKGSFVNGVFEVHKKNVRGEFTYYEIQDNTGKMEVVVHGRLTTINCEEGDKLKLTCFELAPKSGNTGELRSVIHSHIKVIKTRKNKKDILNPDSSMETSPDFFF

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Uniprot Keyword

3D-structure
Acetylation
Activator
Alternative splicing
Apoptosis
Autophagy
Complete proteome
Cytoplasm
DNA-binding
Immunity