Tchem PKN2 Serine/threonine-protein kinase N2

Protein Summary

Description

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. Involved in the negative regulation of ciliogenesis (PubMed:27104747).

Uniprot Accession IDs

• Q16513
• B4DQ21
• B4DTP5
• B4DVG1
• D3DT24
• Q08AF4
• Q9H1W4

Gene Name

• PKN2

Ensembl ID

• ENST00000370513
• ENSP00000359544
• ENSG00000065243
• ENST00000370521
• ENSP00000359552

Symbol

• PRK2
• PRKCL2
• PAK2
• PRK2
• STK7
• Pak-2
• PRKCL2
• PRO2042

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
drug perturbation	1
interacting protein	1
protein domain	0.99
molecular function	0.98
transcription factor binding site profile	0.9

Protein Classes

PANTHER Classes

Protein / Transferase / Kinase / Protein Kinase / Non-receptor Serine/threonine Protein Kinase / Serine/threonine-protein Kinase N2

Protein / Transfer/carrier Protein / Serine/threonine-protein Kinase N2

Protein / Calcium-binding Protein / Intracellular Calcium-sensing Protein / Calmodulin / Serine/threonine-protein Kinase N2

Protein / Calcium-binding Protein / Annexin / Serine/threonine-protein Kinase N2

DTO Classes

Protein / Kinase / Protein Kinase / Agc Group / Pkn Family / Serine/threonine-protein Kinase N2

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 120.45   (req: < 5)

Gene RIFs: 22   (req: <= 3)

Antibodies: 225   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 120.45   (req: >= 5)

Gene RIFs: 22   (req: > 3)

Antibodies: 225   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 25

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 40

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 1

Protein Sequence and Structure

Residue Counts

Protein Sequence

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Expression Data (0 Tissues)

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No expression data found

Related Tools

ProKinO

The ProKinO is a protein kinase-specific ontology, which provides a controlled vocabulary of terms, their hierarchy, and relationships unifying sequence, structure, function, mutation and pathway information on kinases.

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Target Illumination GWAS Analytics (TIGA)

TIGA scores and ranks GWAS discovered associations according to the quantity and quality of the evidence supporting the association.

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GENEVA

GENEVA (GENe Expression Variance Analysis) allows you to identify RNA-sequencing datasets from the Gene Expression Omnibus (GEO) that contain conditions modulating a gene or a gene signature.

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GlyGen

GlyGen is a data integration and dissemination project for carbohydrate and glycoconjugate related data.

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ARCHS4

ARCHS4 provides access to gene-function predictions based on RNA-seq co-expression, and gene expression levels across cell and tissues.

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Approved Drugs (1)

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1 – 1 of 1

midostaurin

PKN2Tchem Kinase

INHIBITOR pKd : 7.96

Active Ligands (40)

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1 – 10 of 40

staurosporine

PKN2Tchem Kinase

pKd : 7.89

lestaurtinib

PKN2Tchem Kinase

pKd : 7.57

SID103905531

PKN2Tchem Kinase

pKi : 9.5

CHEMBL4465866

PKN2Tchem Kinase

pKd : 7.96

CHEMBL4576489

PKN2Tchem Kinase

pKd : 8.22

SID103904754

PKN2Tchem Kinase

pKi : 8.1

SID103904953

PKN2Tchem Kinase

pKi : 7.9

SID103904479

PKN2Tchem Kinase

pKi : 9

SID103904480

PKN2Tchem Kinase

pKi : 8.4

SID103904192

PKN2Tchem Kinase

pKi : 8.3

Protein-Protein Interactions (77)

Explore Interacting Targets

1 – 10 of 77

• See Interactions on String-DB

CETN1

Tbio

Novelty:  0.01208917

p_int:  0.999994588

p_ni:  0.000005412

Score:  0.239

Data Source:  BioPlex,STRINGDB

CAMKV

Tbio

Family:  Kinase

Novelty:  0.17794181

p_int:  0.999641459

p_ni:  0.000358541

Data Source:  BioPlex

FRMPD2

Tbio

Novelty:  0.40302485

p_int:  0.998969254

p_ni:  0.001030746

Score:  0.54

Data Source:  BioPlex,STRINGDB

RHOA

Tbio

Family:  Enzyme

Novelty:  0.00022204

p_int:  0.994655245

p_ni:  0.005344755

Score:  0.994

Data Source:  BioPlex,Reactome,STRINGDB

GOPC

Tbio

Novelty:  0.01110168

p_int:  0.98159675

p_ni:  0.01840325

Score:  0.155

Data Source:  BioPlex,STRINGDB

ZNF785

Tdark

Family:  TF

Novelty:  0.42629766

p_int:  0.964616076

p_ni:  0.035383924

Score:  0.152

Data Source:  BioPlex,STRINGDB

DFFB

Tbio

Novelty:  0.00475349

p_int:  0.947880762

p_ni:  0.052119237

p_wrong:  1e-9

Score:  0.189

Data Source:  BioPlex,STRINGDB

TGM2

Tchem

Family:  Enzyme

Novelty:  0.0005293

p_int:  0.941885559

p_ni:  0.058114441

Data Source:  BioPlex

TGFBR2

Tchem

Family:  Kinase

Novelty:  0.00085381

p_int:  0.876732036

p_ni:  0.123267964

Score:  0.191

Data Source:  BioPlex,STRINGDB

DEF8

Tdark

Novelty:  0.21146806

p_int:  0.862881016

p_ni:  0.137118984

Score:  0.161

Data Source:  BioPlex,STRINGDB

Nearest Tclin Targets

Nearest Tclin calculations are only available for targets with KEGG Pathway annotations.

Pathways (13)

Reactome (4)

KEGG (3)

PathwayCommons (5)

WikiPathways (1)

RHO GTPase Effectors (R-HSA-195258)

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Data Source	Name	Explore in Pharos	Explore in Source
Reactome	RHO GTPase Effectors	273 targets	R-HSA-195258
Reactome	RHO GTPases activate PKNs	63 targets	R-HSA-5625740
Reactome	Signal Transduction	2704 targets	R-HSA-162582
Reactome	Signaling by Rho GTPases	402 targets	R-HSA-194315

Name	Explore in Pharos	Explore in Source
RHO GTPase Effectors	273 targets	R-HSA-195258
RHO GTPases activate PKNs	63 targets	R-HSA-5625740
Signal Transduction	2704 targets	R-HSA-162582
Signaling by Rho GTPases	402 targets	R-HSA-194315

Viral Interactions (0)

No viral interactions found

Gene Ontology Terms (39)

Functions (10)

Components (14)

Processes (15)

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GO Term	Evidence	Assigned by
histone deacetylase binding	Inferred from Direct Assay (IDA)	UniProtKB
kinase activity	Inferred from Direct Assay (IDA)	AgBase
protein serine/threonine kinase activity	Inferred from Direct Assay (IDA)	UniProtKB
RNA polymerase binding	Inferred from Physical Interaction (IPI)	AgBase
cadherin binding	Inferred from High Throughput Direct Assay (HDA)	BHF-UCL
RNA binding	Inferred from High Throughput Direct Assay (HDA)	UniProtKB
protein kinase activity	Traceable Author Statement (TAS)	ProtInc
ATP binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW
GTP-Rho binding	Inferred from Electronic Annotation (IEA)	InterPro
protein kinase C activity	Inferred from Electronic Annotation (IEA)	UniProtKB-EC

Disease Associations ()

No disease associations found

GWAS Traits (16)

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1 – 5 of 16

GWAS Trait	EFO ID	Study Count	SNP Count	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
serum gamma-glutamyl transferase measurement	EFO_0004532	3	3	2	2.8	96
body height	EFO_0004339	3	3	3		92.3
heel bone mineral density	EFO_0009270	3	2	4		87.5
aspartate aminotransferase measurement	EFO_0004736	2	2	1	9.3	85.1
BMI-adjusted waist circumference	EFO_0007789	2	4	5		79.9

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GWAS Trait	EFO ID	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
serum gamma-glutamyl transferase measurement	EFO_0004532	2	2.8	96
body height	EFO_0004339	3		92.3
heel bone mineral density	EFO_0009270	4		87.5
aspartate aminotransferase measurement	EFO_0004736	1	9.3	85.1
BMI-adjusted waist circumference	EFO_0007789	5		79.9

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UniProt Keyword Find Similar Targets

No Header	No Header
3D-structure
Acetylation
Alternative splicing
Apoptosis
ATP-binding
Cell adhesion
Cell cycle
Cell division
Cell junction
Cell projection

GWAS Trait Find Similar Targets

No Header	No Header
aspartate aminotransferase measurement
BMI-adjusted waist circumference
body height
cup-to-disc ratio measurement
heel bone mineral density
hematocrit
hemoglobin measurement
hip circumference
intraocular pressure measurement
lymphocyte count

MGI Phenotype Find Similar Targets

No Header	No Header
behavior/neurological phenotype
cardiovascular system phenotype
cellular phenotype
craniofacial phenotype
embryo phenotype
growth/size/body region phenotype
homeostasis/metabolism phenotype
mortality/aging
nervous system phenotype
respiratory system phenotype

IDG Resources

No IDG generated resources found

Orthologs (12)

1 – 5 of 12

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	protein kinase N2	VGNC:1457	456998
Macaque	protein kinase N2		693505
Mouse	protein kinase N2	MGI:109211	109333
Rat	protein kinase N2	RGD:620146	207122
Dog	protein kinase N2	VGNC:44612	490174

Species	Name	OMA	EggNOG	Inparanoid
Chimp	protein kinase N2
Macaque	protein kinase N2
Mouse	protein kinase N2
Rat	protein kinase N2
Dog	protein kinase N2

Publication Statistics

PubMed Score 120.45

PubMed score by year

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PubTator Score 44.86

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Patents

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Related Publications

Text Mined References (52)

GeneRif Annotations (22)

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PMID	Year	Title