Tbio NONO Non-POU domain-containing octamer-binding protein

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Rna Binding Protein / Mrna Processing Factor / Mrna Splicing Factor / Non-pou Domain-containing Octamer-binding Protein

DTO Classes

Protein / Nucleic Acid Binding / Rna Binding Protein / Mrna Processing Factor / Mrna Splicing Factor / Non-pou Domain-containing Octamer-binding Protein

Protein Summary

Description

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

Uniprot Accession IDs

• Q15233
• B7Z4C2
• D3DVV4
• F5GYZ3
• O00201
• P30807
• Q12786
• Q9BQC5
• NonO protein

Gene Name

• NONO

Ensembl ID

• ENST00000276079
• ENSP00000276079
• ENSG00000147140
• ENST00000373841
• ENSP00000362947
• ENST00000373856
• ENSP00000362963
• ENST00000535149
• ENSP00000441364

Symbol

• NRB54
• P54
• NMT55
• NRB54
• MRXS34
• P54NRB
• PPP1R114

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
co-expressed gene	1
disease perturbation	1
interacting protein	1
ligand (protein) perturbation	1
hub protein	0.96

Related Tools

Target Illumination GWAS Analytics (TIGA)

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GENEVA

GENEVA (GENe Expression Variance Analysis) allows you to identify RNA-sequencing datasets from the Gene Expression Omnibus (GEO) that contain conditions modulating a gene or a gene signature.

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GlyGen

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IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 213.9   (req: < 5)

Gene RIFs: 58   (req: <= 3)

Antibodies: 485   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 213.9   (req: >= 5)

Gene RIFs: 58   (req: > 3)

Antibodies: 485   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 17

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

GWAS Traits (1)

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GWAS Trait	EFO ID	Study Count	SNP Count	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
prostate carcinoma	EFO_0001663	2	1	0	1	10.8

• Explore on Target Illumination GWAS Analytics (TIGA)

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GWAS Trait	EFO ID	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
prostate carcinoma	EFO_0001663	0	1	10.8

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Orthologs (13)

1 – 5 of 13

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	non-POU domain containing octamer binding	VGNC:2912	473661
Macaque	non-POU domain containing octamer binding		697263
Mouse	non-POU-domain-containing, octamer binding protein	MGI:1855692	53610
Rat	non-POU domain containing, octamer-binding	RGD:1549738	317259
Dog	non-POU domain containing octamer binding	VGNC:54178	612773

Species	Name	OMA	EggNOG	Inparanoid
Chimp	non-POU domain containing octamer binding
Macaque	non-POU domain containing octamer binding
Mouse	non-POU-domain-containing, octamer binding protein
Rat	non-POU domain containing, octamer-binding
Dog	non-POU domain containing octamer binding

Protein Structure (2 Structures, 1 AlphaFold Model)

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Representation

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Color Scheme

  target   atomindex   bfactor   chainname   electrostatic   element   hydrophobicity   resname   sstruc

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More information can be found at RCSB PDB

AF-Q15233-F1-model_v1

AlphaFold Structures Developed by DeepMind and EMBL-EBI

1 – 2 of 2

PDB Structure Id	Ligand	Method	Resolution (Å)	Residues	Fraction of Total Protein	Pub Year	Title

PDB Structure Id	Fraction of Total Protein	Resolution	Pub Year

Pathways (4)

PathwayCommons (2)

WikiPathways (2)

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Data Source	Name	Explore in Pharos	Explore in Source
PathwayCommons: netpath	AndrogenReceptor	164 targets	PathwayCommons: netpath
PathwayCommons: pid	Circadian rhythm pathway	17 targets	PathwayCommons: pid

Name	Explore in Pharos	Explore in Source
AndrogenReceptor	164 targets	PathwayCommons: netpath
Circadian rhythm pathway	17 targets	PathwayCommons: pid

Gene Ontology Terms (25)

Functions (7)

Components (8)

Processes (10)

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10

1 – 7 of 7

GO Term	Evidence	Assigned by
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
RNA binding	Inferred from High Throughput Direct Assay (HDA)	UniProtKB
transcription regulatory region sequence-specific DNA binding	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
chromatin binding	Inferred from Sequence or structural Similarity (ISS)	BHF-UCL
E-box binding	Inferred from Sequence or structural Similarity (ISS)	UniProtKB
RNA polymerase II distal enhancer sequence-specific DNA binding	Inferred from Sequence or structural Similarity (ISS)	BHF-UCL
DNA-binding transcription factor activity, RNA polymerase II-specific	Inferred from Sequence Alignment (ISA)	NTNU_SB

Protein-Protein Interactions (193)

Explore Interacting Targets

1 – 10 of 193

• See Interactions on String-DB

TET2

Tbio

Family:  Enzyme

Novelty:  0.00141899

p_int:  0.99999647

p_ni:  0.000003454

p_wrong:  7.5e-8

Score:  0.335

Data Source:  BioPlex,STRINGDB

PROSER1

Tdark

Novelty:  7.63875823

p_int:  0.999994998

p_ni:  6.04e-7

p_wrong:  0.000004398

Score:  0.39

Data Source:  BioPlex,STRINGDB

SFPQ

Tbio

Novelty:  0.01207637

p_int:  0.999931729

p_ni:  0.000068271

Score:  0.999

Data Source:  BioPlex,STRINGDB

GYG2

Tbio

Family:  Enzyme

Novelty:  0.01520991

p_int:  0.99989458

p_ni:  0.000105293

p_wrong:  1.27e-7

Score:  0.177

Data Source:  BioPlex,STRINGDB

PSPC1

Tbio

Novelty:  0.01002246

p_int:  0.998828269

p_ni:  0.001171731

Score:  0.995

Data Source:  BioPlex,STRINGDB

SERPINB7

Tbio

Novelty:  0.01685387

p_int:  0.998408795

p_ni:  0.001591201

p_wrong:  3e-9

Score:  0.173

Data Source:  BioPlex,STRINGDB

SERPINB8

Tbio

Novelty:  0.03308357

p_int:  0.994776172

p_ni:  0.005223828

Score:  0.179

Data Source:  BioPlex,STRINGDB

SERPINA12

Tbio

Novelty:  0.00549132

p_int:  0.992497493

p_ni:  0.007502507

Data Source:  BioPlex

ASAH1

Tchem

Family:  Enzyme

Novelty:  0.00374228

p_int:  0.90328923

p_ni:  0.09671077

Data Source:  BioPlex

CCDC22

Tbio

Novelty:  0.01400901

p_int:  0.895691937

p_ni:  0.104308063

Data Source:  BioPlex

Publication Statistics

PubMed Score  213.90

PubMed score by year

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PubTator Score  109.21

PubTator score by year

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Amino Acid Sequence

Residue Counts

Protein Sequence

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UniProt Keyword Find Similar Targets

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3D-structure
Acetylation
Activator
Alternative splicing
Biological rhythms
Chromosomal rearrangement
Coiled coil
Complete proteome
Direct protein sequencing
DNA damage

GWAS Trait Find Similar Targets

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prostate carcinoma

MGI Phenotype Find Similar Targets

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behavior/neurological phenotype
cellular phenotype
embryo phenotype
homeostasis/metabolism phenotype
liver/biliary system phenotype