Tbio NONO Non-POU domain-containing octamer-binding protein

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Rna Binding Protein / Mrna Processing Factor / Mrna Splicing Factor / Non-pou Domain-containing Octamer-binding Protein

Protein Summary

Description

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

Uniprot Accession IDs

• Q15233
• B7Z4C2
• D3DVV4
• F5GYZ3
• O00201
• P30807
• Q12786
• Q9BQC5
• NonO protein

Gene Name

• NONO

Ensembl ID

• ENST00000276079
• ENSP00000276079
• ENSG00000147140
• ENST00000373841
• ENSP00000362947
• ENST00000373856
• ENSP00000362963
• ENST00000535149
• ENSP00000441364

Symbol

• NRB54
• P54
• NMT55
• NRB54
• MRXS34
• P54NRB
• PPP1R114

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
co-expressed gene	1
disease perturbation	1
interacting protein	1
ligand (protein) perturbation	1
hub protein	0.96

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 529.52   (req: < 5)

Gene RIFs: 58   (req: <= 3)

Antibodies: 485   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 529.52   (req: >= 5)

Gene RIFs: 58   (req: > 3)

Antibodies: 485   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 17

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Data Bank (2)

1 – 2 of 2

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (4)

PathwayCommons (2)

WikiPathways (2)

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Items per page:

5

1 – 2 of 2

Data Source	Name	Explore in Pharos	Explore in Source
PathwayCommons: netpath	AndrogenReceptor	164 targets	PathwayCommons: netpath
PathwayCommons: pid	Circadian rhythm pathway	17 targets	PathwayCommons: pid

Name	Explore in Pharos	Explore in Source
AndrogenReceptor	164 targets	PathwayCommons: netpath
Circadian rhythm pathway	17 targets	PathwayCommons: pid

Gene Ontology Terms (25)

Functions (7)

Components (8)

Processes (10)

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Items per page:

10

1 – 7 of 7

GO Term	Evidence	Assigned by
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
RNA binding	Inferred from High Throughput Direct Assay (HDA)	UniProtKB
transcription regulatory region sequence-specific DNA binding	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
chromatin binding	Inferred from Sequence or structural Similarity (ISS)	BHF-UCL
E-box binding	Inferred from Sequence or structural Similarity (ISS)	UniProtKB
RNA polymerase II distal enhancer sequence-specific DNA binding	Inferred from Sequence or structural Similarity (ISS)	BHF-UCL
DNA-binding transcription factor activity, RNA polymerase II-specific	Inferred from Sequence Alignment (ISA)	NTNU_SB

Protein-Protein Interactions (193)

Explore Interacting Targets

1 – 10 of 193

• See Interactions on String-DB

TET2

Tbio

Family: Enzyme

Novelty: 0.00219804

p_int: 0.99999647

p_ni: 0.000003454

p_wrong: 7.5e-8

Score: 0.335

Data Source: BioPlex,STRINGDB

PROSER1

Tdark

p_int: 0.999994998

p_ni: 6.04e-7

p_wrong: 0.000004398

Score: 0.39

Data Source: BioPlex,STRINGDB

SFPQ

Tbio

Novelty: 0.01546419

p_int: 0.999931729

p_ni: 0.000068271

Score: 0.999

Data Source: BioPlex,STRINGDB

GYG2

Tbio

Family: Enzyme

Novelty: 0.01017368

p_int: 0.99989458

p_ni: 0.000105293

p_wrong: 1.27e-7

Score: 0.177

Data Source: BioPlex,STRINGDB

PSPC1

Tbio

Novelty: 0.01437046

p_int: 0.998828269

p_ni: 0.001171731

Score: 0.995

Data Source: BioPlex,STRINGDB

SERPINB7

Tbio

Novelty: 0.01951612

p_int: 0.998408795

p_ni: 0.001591201

p_wrong: 3e-9

Score: 0.173

Data Source: BioPlex,STRINGDB

SERPINB8

Tbio

Novelty: 0.00412031

p_int: 0.994776172

p_ni: 0.005223828

Score: 0.179

Data Source: BioPlex,STRINGDB

SERPINA12

Tbio

Novelty: 0.00636095

p_int: 0.992497493

p_ni: 0.007502507

Data Source: BioPlex

ASAH1

Tchem

Family: Enzyme

Novelty: 0.00478738

p_int: 0.90328923

p_ni: 0.09671077

Data Source: BioPlex

CCDC22

Tbio

Novelty: 0.01857112

p_int: 0.895691937

p_ni: 0.104308063

Data Source: BioPlex

Publication Statistics

PubMed Score  529.52

PubMed score by year

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PubTator Score  109.21

PubTator score by year

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Amino Acid Sequence

Residue Counts

Protein Sequence

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UniProt Keyword Find Similar Targets

3D-structure
Acetylation
Activator
Alternative splicing
Biological rhythms
Chromosomal rearrangement
Coiled coil
Complete proteome
Direct protein sequencing
DNA damage

MGI Phenotype Find Similar Targets

behavior/neurological phenotype
cellular phenotype
embryo phenotype
homeostasis/metabolism phenotype
liver/biliary system phenotype