Tchem KARS Lysine--tRNA ligase

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Rna Binding Protein / Lysine--trna Ligase

Protein / Ligase / Aminoacyl-trna Synthetase / Lysine--trna Ligase

Protein Summary

Description

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739). (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• Q15046
• A8MSK1
• D3DUK4
• O14946
• Q96J25
• Q9HB23

Gene Name

• KARS

Ensembl ID

• ENST00000302445
• ENSP00000303043
• ENSG00000065427
• ENST00000319410
• ENSP00000325448

Symbol

• KIAA0070
• KRS
• KARS1
• KARS2
• CMTRIB
• DFNB89

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
co-expressed gene	1
interacting protein	1
kinase perturbation	1
molecular function	0.98
protein domain	0.92

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 304.17   (req: < 5)

Gene RIFs: 33   (req: <= 3)

Antibodies: 202   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 304.17   (req: >= 5)

Gene RIFs: 33   (req: > 3)

Antibodies: 202   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 26

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 45

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Active Ligands (45)

Explore Active Ligands

1 – 10 of 45

CHEMBL388978

KARSTchem Enzyme

IC50: 8.05

CHEMBL509032

KARSTchem Enzyme

IC50: 6.54

CHEMBL2178352

KARSTchem Enzyme

IC50: 6.14

CHEMBL459850

KARSTchem Enzyme

EC50: 7.15

CHEMBL1835870

KARSTchem Enzyme

Ki: 6.66

CHEMBL282342

KARSTchem Enzyme

IC50: 7.47

CHEMBL471901

KARSTchem Enzyme

IC50: 7.39

CHEMBL475609

KARSTchem Enzyme

IC50: 8.05

CHEMBL475575

KARSTchem Enzyme

IC50: 8.4

CHEMBL474582

KARSTchem Enzyme

IC50: 8.05

Protein Data Bank (5)

1 – 5 of 5

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (10)

Reactome (8)

KEGG (1)

PathwayCommons (1)

Cytosolic tRNA aminoacylation (R-HSA-379716)

Click on a row in the table to change the structure displayed.

Items per page:

5

1 – 5 of 8

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Cytosolic tRNA aminoacylation	24 targets	R-HSA-379716
Reactome	Metabolism	2112 targets	R-HSA-1430728
Reactome	Metabolism of amino acids and derivatives	373 targets	R-HSA-71291
Reactome	Metabolism of proteins	2050 targets	R-HSA-392499
Reactome	Mitochondrial tRNA aminoacylation	21 targets	R-HSA-379726

Name	Explore in Pharos	Explore in Source
Cytosolic tRNA aminoacylation	24 targets	R-HSA-379716
Metabolism	2112 targets	R-HSA-1430728
Metabolism of amino acids and derivatives	373 targets	R-HSA-71291
Metabolism of proteins	2050 targets	R-HSA-392499
Mitochondrial tRNA aminoacylation	21 targets	R-HSA-379726

Gene Ontology Terms (33)

Functions (7)

Components (7)

Processes (19)

Items per page:

10

1 – 7 of 7

GO Term	Evidence	Assigned by
ATP adenylyltransferase activity	Inferred from Direct Assay (IDA)	UniProtKB
lysine-tRNA ligase activity	Inferred from Direct Assay (IDA)	UniProtKB
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
protein homodimerization activity	Inferred from Physical Interaction (IPI)	UniProtKB
tRNA binding	Non-traceable Author Statement (NAS)	UniProtKB
amino acid binding	Inferred from Electronic Annotation (IEA)	Ensembl
ATP binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW

Protein-Protein Interactions (303)

Explore Interacting Targets

1 – 10 of 303

• See Interactions on String-DB

RARS

Tchem

Family: Enzyme

Novelty: 0.00081224

p_int: 0.999994549

p_ni: 0.000005451

Score: 0.999

Data Source: BioPlex,STRINGDB

TARSL2

Tbio

Family: Enzyme

Novelty: 0.0300025

p_int: 0.999993971

p_ni: 0.000006029

Score: 0.969

Data Source: BioPlex,STRINGDB

SDCCAG8

Tbio

Novelty: 0.06798507

p_int: 0.999988727

p_ni: 0.000011152

p_wrong: 1.21e-7

Score: 0.741

Data Source: BioPlex,STRINGDB

AIMP2

Tbio

Family: Enzyme

Novelty: 0.03974199

p_int: 0.999982175

p_ni: 0.000017825

Score: 0.999

Data Source: BioPlex,STRINGDB

AIMP1

Tbio

Family: Enzyme

Novelty: 0.00828433

p_int: 0.9996742

p_ni: 0.0003258

Score: 0.998

Data Source: BioPlex,STRINGDB

DARS

Tbio

Family: Enzyme

Novelty: 0.01186323

p_int: 0.999149756

p_ni: 0.000850244

Score: 0.994

Data Source: BioPlex,STRINGDB

EPRS

Tchem

Family: Enzyme

Novelty: 0.00072148

p_int: 0.998884029

p_ni: 0.001115971

Score: 0.999

Data Source: BioPlex,STRINGDB

EEF1E1

Tbio

Novelty: 0.07766287

p_int: 0.998835167

p_ni: 0.001164833

Score: 0.994

Data Source: BioPlex,STRINGDB

IARS

Tchem

Family: Enzyme

Novelty: 0.0033571

p_int: 0.998494698

p_ni: 0.001505302

Score: 0.999

Data Source: BioPlex,STRINGDB

LARS

Tchem

Family: Enzyme

Novelty: 0.00167582

p_int: 0.998340387

p_ni: 0.001659613

Score: 0.998

Data Source: BioPlex,STRINGDB

Publication Statistics

PubMed Score  304.17

PubMed score by year

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PubTator Score  138.5

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEES

1-70

VDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGG

70-140

KLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCL

140-210

HMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVA

210-280

KPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDL

280-350

MEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETR

350-420

KILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFV

420-490

MKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTD

490-560

SNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

560-597

MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

Find similar targets by:

Uniprot Keyword

3D-structure
Acetylation
Alternative splicing
Aminoacyl-tRNA synthetase
ATP-binding
Cell membrane
Charcot-Marie-Tooth disease
Complete proteome
Cytoplasm
Deafness