Tbio EIF3A Eukaryotic translation initiation factor 3 subunit A

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Rna Binding Protein / Translation Factor / Translation Initiation Factor / Eukaryotic Translation Initiation Factor 3 Subunit A

DTO Classes

Protein / Nucleic Acid Binding / Rna Binding Protein / Translation Factor / Translation Initiation Factor / Eukaryotic Translation Initiation Factor 3 Subunit A

Protein Summary

Description

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815). (Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).

Uniprot Accession IDs

• Q14152
• B1AMV5
• B4DYS1
• F5H335
• O00653
• Q15778
• eIF3a

Gene Name

• EIF3A

Ensembl ID

• ENST00000369144
• ENSP00000358140
• ENSG00000107581
• ENST00000541549
• ENSP00000438178

Symbol

• EIF3S10
• KIAA0139
• EIF3
• P167
• p180
• p185
• TIF32
• EIF3S10
• eIF3-p170
• eIF3-theta

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
disease perturbation	1
transcription factor perturbation	0.96
hub protein	0.88
virus perturbation	0.88
transcription factor binding site profile	0.86

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 184.62   (req: < 5)

Gene RIFs: 48   (req: <= 3)

Antibodies: 252   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 184.62   (req: >= 5)

Gene RIFs: 48   (req: > 3)

Antibodies: 252   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 11

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Data Bank (2)

1 – 2 of 2

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (14)

Reactome (11)

KEGG (1)

PathwayCommons (1)

WikiPathways (1)

Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S (R-HSA-72662)

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Items per page:

5

1 – 5 of 11

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S	61 targets	R-HSA-72662
Reactome	Cap-dependent Translation Initiation	121 targets	R-HSA-72737
Reactome	Eukaryotic Translation Initiation	121 targets	R-HSA-72613
Reactome	Formation of a pool of free 40S subunits	103 targets	R-HSA-72689
Reactome	Formation of the ternary complex, and subsequently, the 43S complex	53 targets	R-HSA-72695

Name	Explore in Pharos	Explore in Source
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S	61 targets	R-HSA-72662
Cap-dependent Translation Initiation	121 targets	R-HSA-72737
Eukaryotic Translation Initiation	121 targets	R-HSA-72613
Formation of a pool of free 40S subunits	103 targets	R-HSA-72689
Formation of the ternary complex, and subsequently, the 43S complex	53 targets	R-HSA-72695

Gene Ontology Terms (24)

Functions (5)

Components (13)

Processes (6)

Items per page:

10

1 – 5 of 5

GO Term	Evidence	Assigned by
RNA binding	Inferred from Direct Assay (IDA)	UniProtKB
mRNA binding	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
structural molecule activity	Non-traceable Author Statement (NAS)	UniProtKB
receptor tyrosine kinase binding	Inferred from Electronic Annotation (IEA)	Ensembl
translation initiation factor activity	Inferred from Electronic Annotation (IEA)	UniProtKB-UniRule

Protein-Protein Interactions (324)

Explore Interacting Targets

1 – 10 of 324

• See Interactions on String-DB

EIF3E

Tbio

Novelty: 0.02690754

p_int: 0.999995496

p_ni: 0.000004504

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3M

Tbio

Novelty: 0.02340046

p_int: 0.999991829

p_ni: 0.000008171

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3H

Tbio

Novelty: 0.03294676

p_int: 0.999990805

p_ni: 0.000009195

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3I

Tbio

Novelty: 0.07521172

p_int: 0.999989646

p_ni: 0.000010354

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3D

Tbio

Novelty: 0.05637435

p_int: 0.999974041

p_ni: 0.000025959

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3L

Tbio

Novelty: 0.1477297

p_int: 0.999973584

p_ni: 0.000026416

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3F

Tbio

Novelty: 0.03659119

p_int: 0.999936373

p_ni: 0.000063627

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF3G

Tbio

Novelty: 0.06880717

p_int: 0.999847015

p_ni: 0.000152985

Score: 0.999

Data Source: BioPlex,STRINGDB

EIF4A1

Tchem

Novelty: 0.0043238

p_int: 0.992818894

p_ni: 0.007181106

Score: 0.988

Data Source: BioPlex,STRINGDB

RSPH9

Tbio

Novelty: 0.12094399

p_int: 0.983007521

p_ni: 0.016992479

Score: 0.722

Data Source: BioPlex,STRINGDB

Publication Statistics

PubMed Score  184.62

PubMed score by year

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PubTator Score  329.94

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEG

1-70

LYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDR

70-140

TDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQR

140-210

HHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVS

210-280

TVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVE

280-350

KQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEK

350-420

EPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRT

420-490

LSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKN

490-560

SRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQ

560-630

IKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFER

630-700

AKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQS

700-770

VYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEE

770-840

LREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWR

840-910

RGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRR

910-980

GADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRR

980-1050

GGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPW

1050-1120

RNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWG

1120-1190

PPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWR

1190-1260

DSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRV

1260-1330

PPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

1330-1382

MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

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Uniprot Keyword

3D-structure
Acetylation
Alternative splicing
Coiled coil
Complete proteome
Cytoplasm
Direct protein sequencing
Initiation factor
Phosphoprotein
Polymorphism