Tbio CUL4B Cullin-4B

Protein Classes

PANTHER Classes

Protein / Ligase / Ubiquitin-protein Ligase / Cullin-4b

DTO Classes

Protein / Enzyme / Ligase / Ubiquitin-protein Ligase / Cullin-4b

Protein Summary

Description

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• Q13620
• B1APK5
• B3KVX4
• B7Z5K8
• Q6PIE4
• Q6UP07
• Q7Z673
• Q9BY37
• Q9UEB7
• Q9UED7
• CUL-4B

Gene Name

• CUL4B

Ensembl ID

• ENST00000371322
• ENSP00000360373
• ENSG00000158290
• ENST00000404115
• ENSP00000384109

Symbol

• KIAA0695
• SFM2
• MRXSC
• CUL-4B
• MRXHF2
• MRXS15

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
transcription factor perturbation	0.97
protein domain	0.85
biological process	0.71
tissue	0.67
tissue sample	0.67

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 63.25   (req: < 5)

Gene RIFs: 46   (req: <= 3)

Antibodies: 351   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 63.25   (req: >= 5)

Gene RIFs: 46   (req: > 3)

Antibodies: 351   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 20

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Data Bank (4)

1 – 4 of 4

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (56)

Reactome (15)

KEGG (3)

UniProt (1)

WikiPathways (37)

DNA Damage Bypass (R-HSA-73893)

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Items per page:

5

1 – 5 of 15

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	DNA Damage Bypass	47 targets	R-HSA-73893
Reactome	DNA Damage Recognition in GG-NER	38 targets	R-HSA-5696394
Reactome	DNA Repair	306 targets	R-HSA-73894
Reactome	Dual Incision in GG-NER	41 targets	R-HSA-5696400
Reactome	Dual incision in TC-NER	65 targets	R-HSA-6782135

Name	Explore in Pharos	Explore in Source
DNA Damage Bypass	47 targets	R-HSA-73893
DNA Damage Recognition in GG-NER	38 targets	R-HSA-5696394
DNA Repair	306 targets	R-HSA-73894
Dual Incision in GG-NER	41 targets	R-HSA-5696400
Dual incision in TC-NER	65 targets	R-HSA-6782135

Gene Ontology Terms (26)

Functions (1)

Components (6)

Processes (19)

Items per page:

10

1 – 1 of 1

GO Term	Evidence	Assigned by
ubiquitin protein ligase binding	Inferred from Biological aspect of Ancestor (IBA)	GO_Central

Protein-Protein Interactions (301)

Explore Interacting Targets

1 – 10 of 301

• See Interactions on String-DB

TRPC4AP

Tbio

Novelty: 0.00440674

p_int: 0.999802604

p_ni: 0.000197396

Score: 0.92

Data Source: BioPlex,STRINGDB

DCAF4

Tdark

Novelty: 0.32846715

p_int: 0.999774038

p_ni: 0.000225962

Score: 0.984

Data Source: BioPlex,STRINGDB

DDB2

Tbio

Novelty: 0.00809774

p_int: 0.999747729

p_ni: 0.000252271

Score: 0.993

Data Source: BioPlex,Reactome,STRINGDB

DCAF11

Tbio

Novelty: 0.12689461

p_int: 0.999727015

p_ni: 0.000272985

Score: 0.981

Data Source: BioPlex,STRINGDB

DCAF8

Tbio

Novelty: 0.27272727

p_int: 0.99969849

p_ni: 0.00030151

Score: 0.946

Data Source: BioPlex,STRINGDB

GLMN

Tbio

Novelty: 0.01373749

p_int: 0.999631709

p_ni: 0.000368291

Score: 0.793

Data Source: BioPlex,STRINGDB

WDTC1

Tbio

Novelty: 0.00004184

p_int: 0.999613118

p_ni: 0.000386882

Score: 0.93

Data Source: BioPlex,STRINGDB

COPS8

Tbio

Novelty: 0.05324769

p_int: 0.999293628

p_ni: 0.000706372

Score: 0.979

Data Source: BioPlex,STRINGDB

DCAF8L2

Tdark

p_int: 0.999271641

p_ni: 0.000728359

Score: 0.158

Data Source: BioPlex,STRINGDB

DET1

Tbio

Novelty: 0.01807423

p_int: 0.999108581

p_ni: 0.000891419

Score: 0.985

Data Source: BioPlex,STRINGDB

Publication Statistics

PubMed Score  63.25

PubMed score by year

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PubTator Score  49.08

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSS

1-70

NEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSS

70-140

SSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPE

140-210

NYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSL

210-280

DSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGI

280-350

LLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKR

350-420

LEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVL

420-490

LQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPA

490-560

ELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSML

560-630

SKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPE

630-700

MVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQA

700-770

TGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTE

770-840

RVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYN

840-910

YIA

910-913

MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA

Find similar targets by:

Uniprot Keyword

3D-structure
Acetylation
Alternative splicing
Cell cycle
Complete proteome
Disease mutation
DNA damage
DNA repair
Dwarfism
Isopeptide bond