Tbio PUF60 Poly(U)-binding-splicing factor PUF60

Protein Summary

Description

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA. This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Accession IDs

• Q9UHX1
• A8K8K8
• Q969E7
• Q96D94
• Q96H63
• Q99628
• Q9NZA0
• Q9UJY7

Gene Name

• PUF60

Ensembl ID

• ENST00000313352
• ENSP00000322016
• ENSG00000179950
• ENST00000349157
• ENSP00000322036
• ENST00000453551
• ENSP00000402953
• ENST00000456095
• ENSP00000395417
• ENST00000526683
• ENSP00000434359
• ENST00000527197
• ENSP00000431960
• ENST00000612237
• ENSP00000478495
• ENSG00000274081
• ENST00000620341
• ENSP00000484860
• ENST00000620953
• ENSP00000478209
• ENST00000632444
• ENSP00000487945
• ENST00000633206
• ENSP00000487930

Symbol

• FIR
• ROBPI
• SIAHBP1
• FIR
• VRJS
• RoBPI
• SIAHBP1

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
disease perturbation	1
interacting protein	1
kinase perturbation	1
co-expressed gene	0.99
transcription factor binding site profile	0.87

Protein Classes

No PANTHER Classes or DTO Classes found

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 49.94   (req: < 5)

Gene RIFs: 27   (req: <= 3)

Antibodies: 413   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 49.94   (req: >= 5)

Gene RIFs: 27   (req: > 3)

Antibodies: 413   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 6

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0