Tbio POLR2A DNA-directed RNA polymerase II subunit RPB1

Protein Summary

Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity). (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P24928
• A6NN93
• B9EH88
• Q6NX41
• RNA polymerase II subunit B1

Gene Name

• POLR2A

Ensembl ID

• ENST00000572844
• ENSP00000461879
• ENSG00000181222

Symbol

• POLR2
• RPB1
• RPO2
• POLR2
• POLRA
• RPBh1
• RPOL2
• RpIILS
• hsRPB1
• hRPB220

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
hub protein	1
co-expressed gene	0.99
kinase	0.99
phosphatase	0.99
protein domain	0.99

Protein Classes

PANTHER Classes

Protein / Transferase / Nucleotidyltransferase / Dna-directed Rna Polymerase Ii Subunit Rpb1

Protein / Nucleic Acid Binding / Rna Binding Protein / Dna-directed Rna Polymerase / Dna-directed Rna Polymerase Ii Subunit Rpb1

DTO Classes

Protein / Enzyme / Transferase / Nucleotidyltransferase / Polymerase / Dna-directed Rna Polymerase / Dna-directed Rna Polymerase Ii Subunit Rpb1

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 654.22   (req: < 5)

Gene RIFs: 112   (req: <= 3)

Antibodies: 716   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 654.22   (req: >= 5)

Gene RIFs: 112   (req: > 3)

Antibodies: 716   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 21

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0