Tbio DAD1 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Protein Summary

Description

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853). DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P61803
• D3DS25
• O08552
• O70364
• P46966
• P46968
• Q6FGA3
• Q96GB7
• Oligosaccharyl transferase subunit DAD1

Gene Name

• DAD1

Ensembl ID

• ENST00000250498
• ENSP00000250498
• ENSG00000129562

Symbol

• OST2

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
disease perturbation	0.87
transcription factor binding site profile	0.86
virus perturbation	0.85
kinase perturbation	0.84
cell type or tissue	0.79

Protein Classes

PANTHER Classes

Protein / Transferase / Glycosyltransferase / Dolichyl-diphosphooligosaccharide--protein Glycosyltransferase Subunit Dad1

DTO Classes

Protein / Enzyme / Transferase / Glycosyltransferase / Dolichyl-diphosphooligosaccharide--protein Glycosyltransferase Subunit Dad1

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 160.62   (req: < 5)

Gene RIFs: 8   (req: <= 3)

Antibodies: 251   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 160.62   (req: >= 5)

Gene RIFs: 8   (req: > 3)

Antibodies: 251   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 9

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0