Tbio RAB8A Ras-related protein Rab-8A

Protein Summary

Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). Plays an important role in ciliogenesis (PubMed:21844891). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069). The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P61006
• B4DEK7
• P24407
• Q6FHV5

Gene Name

• RAB8A

Ensembl ID

• ENST00000300935
• ENSP00000300935
• ENSG00000167461
• ENST00000586682
• ENSP00000467501

Symbol

• MEL
• RAB8
• MEL
• RAB8

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
kinase perturbation	1
molecular function	0.99
virus perturbation	0.99
disease perturbation	0.97
interacting protein	0.96

Related Tools

Target Illumination GWAS Analytics (TIGA)

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GENEVA

GENEVA (GENe Expression Variance Analysis) allows you to identify RNA-sequencing datasets from the Gene Expression Omnibus (GEO) that contain conditions modulating a gene or a gene signature.

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GlyGen

GlyGen is a data integration and dissemination project for carbohydrate and glycoconjugate related data.

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ARCHS4

ARCHS4 provides access to gene-function predictions based on RNA-seq co-expression, and gene expression levels across cell and tissues.

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IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 185.18   (req: < 5)

Gene RIFs: 53   (req: <= 3)

Antibodies: 251   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 185.18   (req: >= 5)

Gene RIFs: 53   (req: > 3)

Antibodies: 251   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 24

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

GWAS Traits (3)

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GWAS Trait	EFO ID	Study Count	SNP Count	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
red blood cell distribution width	EFO_0005192	2	2	2		76.7
mean corpuscular hemoglobin concentration	EFO_0004528	1	1	1		61.2
mean corpuscular hemoglobin	EFO_0004527	1	1	1		27.2

• Explore on Target Illumination GWAS Analytics (TIGA)

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GWAS Trait	EFO ID	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
red blood cell distribution width	EFO_0005192	2		76.7
mean corpuscular hemoglobin concentration	EFO_0004528	1		61.2
mean corpuscular hemoglobin	EFO_0004527	1		27.2

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Orthologs (12)

1 – 5 of 12

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	RAB8A, member RAS oncogene family	VGNC:7571	468760
Macaque	RAB8A, member RAS oncogene family		100423251
Mouse	RAB8A, member RAS oncogene family	MGI:96960	17274
Rat	RAB8A, member RAS oncogene family	RGD:621144	117103
Dog	RAB8A, member RAS oncogene family	VGNC:45295	403773

Species	Name	OMA	EggNOG	Inparanoid
Chimp	RAB8A, member RAS oncogene family
Macaque	RAB8A, member RAS oncogene family
Mouse	RAB8A, member RAS oncogene family
Rat	RAB8A, member RAS oncogene family
Dog	RAB8A, member RAS oncogene family

Protein Structure (9 Structures, 1 AlphaFold Model)

Representation

  axes   backbone   ball+stick   cartoon   rocket   rope   spacefill

Color Scheme

  target   atomindex   bfactor   chainname   electrostatic   element   hydrophobicity   resname   sstruc

Click on a row in the table to change the structure displayed.
More information can be found at RCSB PDB

AF-P61006-F1-model_v1

AlphaFold Structures Developed by DeepMind and EMBL-EBI

1 – 5 of 9

PDB Structure Id	Ligand	Method	Resolution (Å)	Residues	Fraction of Total Protein	Pub Year	Title

PDB Structure Id	Fraction of Total Protein	Resolution	Pub Year

Pathways (120)

Reactome (19)

KEGG (5)

PathwayCommons (1)

WikiPathways (95)

Anchoring of the basal body to the plasma membrane (R-HSA-5620912)

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Find Similar Targets

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Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Anchoring of the basal body to the plasma membrane	97 targets	R-HSA-5620912
Reactome	Cargo trafficking to the periciliary membrane	51 targets	R-HSA-5620920
Reactome	Cell Cycle	597 targets	R-HSA-1640170
Reactome	Cell Cycle, Mitotic	495 targets	R-HSA-69278
Reactome	Cilium Assembly	187 targets	R-HSA-5617833

Name	Explore in Pharos	Explore in Source
Anchoring of the basal body to the plasma membrane	97 targets	R-HSA-5620912
Cargo trafficking to the periciliary membrane	51 targets	R-HSA-5620920
Cell Cycle	597 targets	R-HSA-1640170
Cell Cycle, Mitotic	495 targets	R-HSA-69278
Cilium Assembly	187 targets	R-HSA-5617833

Gene Ontology Terms (46)

Functions (6)

Components (22)

Processes (18)

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1 – 6 of 6

GO Term	Evidence	Assigned by
GDP binding	Inferred from Direct Assay (IDA)	UniProtKB
GTP binding	Inferred from Direct Assay (IDA)	UniProtKB
GTPase activity	Inferred from Direct Assay (IDA)	UniProtKB
myosin V binding	Inferred from Physical Interaction (IPI)	UniProtKB
Rab GTPase binding	Inferred from Sequence or structural Similarity (ISS)	UniProtKB
protein kinase binding	Inferred from Electronic Annotation (IEA)	Ensembl

Protein-Protein Interactions (360)

Explore Interacting Targets

1 – 10 of 360

• See Interactions on String-DB

RAB8B

Tbio

Family: Enzyme

Novelty: 0.06230082

p_int: 0.999999999

p_ni: 1e-9

Score: 0.971

Data Source: BioPlex,STRINGDB

GDI1

Tbio

Novelty: 0.01710945

p_int: 0.999999788

p_ni: 2.12e-7

Score: 0.994

Data Source: BioPlex,Reactome,STRINGDB

RABIF

Tbio

Novelty: 0.22512579

p_int: 0.999999186

p_ni: 8.14e-7

Score: 0.984

Data Source: BioPlex,STRINGDB

RAB1A

Tbio

Family: Enzyme

Novelty: 0.00307527

p_int: 0.999385637

p_ni: 0.00061435

p_wrong: 1.3e-8

Score: 0.983

Data Source: BioPlex,STRINGDB

SRPRB

Tbio

Novelty: 0.04735362

p_int: 0.996649295

p_ni: 0.003350685

p_wrong: 2e-8

Data Source: BioPlex

RPH3A

Tbio

Family: Epigenetic

Novelty: 0.01321524

p_int: 0.992442008

p_ni: 0.007557987

p_wrong: 5e-9

Score: 0.796

Data Source: BioPlex,STRINGDB

RAB3IL1

Tbio

Novelty: 0.0167537

p_int: 0.971654395

p_ni: 0.02832901

p_wrong: 0.000016594

Score: 0.977

Data Source: BioPlex,STRINGDB

SLC1A1

Tchem

Family: Transporter

Novelty: 0.00218479

p_int: 0.8213597

p_ni: 0.178639363

p_wrong: 9.37e-7

Score: 0.207

Data Source: BioPlex,STRINGDB

GDI2

Tbio

Novelty: 0.02861013

Score: 0.995

Data Source: Reactome,STRINGDB

RAB3IP

Tbio

Novelty: 0.04046982

Score: 0.993

Data Source: Reactome,STRINGDB

Publication Statistics

PubMed Score  185.18

PubMed score by year

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PubTator Score  98.19

PubTator score by year

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Amino Acid Sequence

Residue Counts

Protein Sequence

Show Full Sequence

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Find similar targets by:

UniProt Keyword Find Similar Targets

3D-structure
Alternative splicing
Autophagy
Cell membrane
Cell projection
Cilium
Cilium biogenesis/degradation
Complete proteome
Cytoplasm
Cytoplasmic vesicle

GWAS Trait Find Similar Targets

mean corpuscular hemoglobin
mean corpuscular hemoglobin concentration
red blood cell distribution width

MGI Phenotype Find Similar Targets

adipose tissue phenotype
cellular phenotype
digestive/alimentary phenotype
growth/size/body region phenotype
mortality/aging
renal/urinary system phenotype