Tchem FEN1 Flap endonuclease 1

Protein Summary

Description

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P39748
• FEN-1

Gene Name

• FEN1

Ensembl ID

• ENST00000305885
• ENSP00000305480
• ENSG00000168496

Symbol

• RAD2
• MF1
• RAD2
• FEN-1

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
interacting protein	0.99
molecular function	0.98
kinase perturbation	0.97
co-expressed gene	0.93
protein domain	0.92

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Nuclease / Exodeoxyribonuclease / Flap Endonuclease 1

Protein / Nucleic Acid Binding / Nuclease / Endodeoxyribonuclease / Flap Endonuclease 1

Protein / Nucleic Acid Binding / Dna Binding Protein / Exodeoxyribonuclease / Flap Endonuclease 1

Protein / Nucleic Acid Binding / Dna Binding Protein / Endodeoxyribonuclease / Flap Endonuclease 1

Protein / Nucleic Acid Binding / Dna Binding Protein / Damaged Dna-binding Protein / Flap Endonuclease 1

Protein / Hydrolase / Flap Endonuclease 1

DTO Classes

Protein / Enzyme / Nuclease / Exodeoxyribonuclease / Flap Endonuclease 1

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 1483.02   (req: < 5)

Gene RIFs: 142   (req: <= 3)

Antibodies: 522   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 1483.02   (req: >= 5)

Gene RIFs: 142   (req: > 3)

Antibodies: 522   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 26

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 35

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0