Tchem MCM4 DNA replication licensing factor MCM4

Protein Summary

Description

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P33991
• Q8NEH1
• Q99658

Gene Name

• MCM4

Ensembl ID

• ENST00000262105
• ENSP00000262105
• ENSG00000104738
• ENST00000523944
• ENSP00000430194
• ENST00000649973
• ENSP00000496964

Symbol

• CDC21
• NKCD
• CDC21
• CDC54
• IMD54
• NKGCD
• hCdc21
• P1-CDC21

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
molecular function	0.99
transcription factor perturbation	0.99
protein domain	0.97
kinase perturbation	0.91
histone modification site profile	0.9

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Helicase / Dna Helicase / Dna Replication Licensing Factor Mcm4

Protein / Nucleic Acid Binding / Dna Binding Protein / Dna Helicase / Dna Replication Licensing Factor Mcm4

Protein / Hydrolase / Dna Replication Licensing Factor Mcm4

DTO Classes

Protein / Enzyme / Helicase / Dna Helicase / Dna Replication Licensing Factor Mcm4

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 194.41   (req: < 5)

Gene RIFs: 34   (req: <= 3)

Antibodies: 413   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 194.41   (req: >= 5)

Gene RIFs: 34   (req: > 3)

Antibodies: 413   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 7

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 1

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0