Tchem WEE1 Wee1-like protein kinase

Protein Classes

PANTHER Classes

Protein / Transferase / Kinase / Protein Kinase / Non-receptor Serine/threonine Protein Kinase / Wee1-like Protein Kinase

DTO Classes

Protein / Kinase / Protein Kinase / Other Group / Wee Family / Wee1-like Protein Kinase

Protein Summary

Description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P30291
• B3KVE1
• D3DQV0
• WEE1hu

Gene Name

• WEE1

Ensembl ID

• ENST00000299613
• ENSP00000299613
• ENSG00000166483
• ENST00000450114
• ENSP00000402084

Symbol

• WEE1A
• WEE1hu

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
molecular function	0.96
PubMedID	0.94
transcription factor binding site profile	0.92
hub protein	0.9
kinase	0.88

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 463.07   (req: < 5)

Gene RIFs: 85   (req: <= 3)

Antibodies: 594   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 463.07   (req: >= 5)

Gene RIFs: 85   (req: > 3)

Antibodies: 594   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 13

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 72

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Active Ligands (72)

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1 – 10 of 72

CHEMBL49120

WEE1Tchem Kinase

IC50: 7.61999989

CHEMBL272479

WEE1Tchem Kinase

IC50: 7.59

CHEMBL411662

WEE1Tchem Kinase

IC50: 8.05

CHEMBL255565,CHEMBL469612

WEE1Tchem Kinase

IC50: 7.62

CHEMBL258067

WEE1Tchem Kinase

IC50: 7.72

CHEMBL379975

WEE1Tchem Kinase

IC50: 7.96

CHEMBL213593

WEE1Tchem Kinase

IC50: 7.55

CHEMBL271715

WEE1Tchem Kinase

IC50: 7.82

CHEMBL515352

WEE1Tchem Kinase

IC50: 8.15

CHEMBL488054

WEE1Tchem Kinase

IC50: 7.57

Protein Data Bank (20)

1 – 5 of 20

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (70)

Reactome (18)

KEGG (2)

PathwayCommons (6)

WikiPathways (44)

Cell Cycle (R-HSA-1640170)

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Items per page:

5

1 – 5 of 18

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Cell Cycle	597 targets	R-HSA-1640170
Reactome	Cell Cycle Checkpoints	272 targets	R-HSA-69620
Reactome	Cell Cycle, Mitotic	495 targets	R-HSA-69278
Reactome	Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex	13 targets	R-HSA-75035
Reactome	Cyclin A/B1/B2 associated events during G2/M transition	25 targets	R-HSA-69273

Name	Explore in Pharos	Explore in Source
Cell Cycle	597 targets	R-HSA-1640170
Cell Cycle Checkpoints	272 targets	R-HSA-69620
Cell Cycle, Mitotic	495 targets	R-HSA-69278
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex	13 targets	R-HSA-75035
Cyclin A/B1/B2 associated events during G2/M transition	25 targets	R-HSA-69273

Gene Ontology Terms (17)

Functions (6)

Components (4)

Processes (7)

Items per page:

10

1 – 6 of 6

GO Term	Evidence	Assigned by
protein kinase activity	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
protein tyrosine kinase activity	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
kinase activity	Traceable Author Statement (TAS)	Reactome
ATP binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW
magnesium ion binding	Inferred from Electronic Annotation (IEA)	InterPro
non-membrane spanning protein tyrosine kinase activity	Inferred from Electronic Annotation (IEA)	UniProtKB-EC

Protein-Protein Interactions (203)

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1 – 10 of 203

• See Interactions on String-DB

YEATS4

Tbio

Novelty: 0.0323643

p_int: 0.999997788

p_ni: 0.000002032

p_wrong: 1.8e-7

Score: 0.211

Data Source: BioPlex,STRINGDB

CDK1

Tchem

Family: Kinase

Novelty: 0.00049984

Score: 0.998

Data Source: Reactome,STRINGDB

CDC25C

Tchem

Family: Enzyme

Novelty: 0.00287182

Score: 0.99

Data Source: STRINGDB

CDC25A

Tchem

Family: Enzyme

Novelty: 0.00322177

Score: 0.987

Data Source: STRINGDB

CCNB1

Tchem

Novelty: 0.00068627

Score: 0.987

Data Source: Reactome,STRINGDB

CCNA2

Tchem

Novelty: 0.00061948

Score: 0.98

Data Source: STRINGDB

CDK2

Tchem

Family: Kinase

Novelty: 0.00033882

Score: 0.973

Data Source: Reactome,STRINGDB

PLK1

Tchem

Family: Kinase

Novelty: 0.00071387

Score: 0.97

Data Source: Reactome,STRINGDB

CCNE1

Tchem

Novelty: 0.00296958

Score: 0.964

Data Source: STRINGDB

CCNE2

Tbio

Novelty: 0.01296696

Score: 0.962

Data Source: STRINGDB

Publication Statistics

PubMed Score  463.07

PubMed score by year

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PubTator Score  258.77

PubTator score by year

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Patents

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Amino Acid Sequence

Residue Counts

Sequence

MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTE

1-70

PGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSP

70-140

VRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRG

140-210

SSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRP

210-280

AKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAH

280-350

AVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSM

350-420

SLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQE

420-490

NYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSA

490-560

MALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTS

560-630

RLIGKKMNRSVSLTIY

630-646

MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Find similar targets by:

Uniprot Keyword

3D-structure
Alternative splicing
ATP-binding
Cell cycle
Cell division
Complete proteome
Kinase
Magnesium
Metal-binding
Mitosis