Tbio CDC27 Cell division cycle protein 27 homolog

Protein Summary

Description

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

Uniprot Accession IDs

• P30260
• G3V1C4
• Q16349
• Q96F35

Gene Name

• CDC27

Ensembl ID

• ENST00000066544
• ENSP00000066544
• ENSG00000004897
• ENST00000531206
• ENSP00000434614

Symbol

• ANAPC3
• D0S1430E
• D17S978E
• APC3
• HNUC
• NUC2
• H-NUC
• ANAPC3
• CDC27Hs
• D0S1430E
• D17S978E

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
hub protein	0.99
transcription factor perturbation	0.96
biological process	0.94
interacting protein	0.94
histone modification site profile	0.92

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 98.81   (req: < 5)

Gene RIFs: 18   (req: <= 3)

Antibodies: 452   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 98.81   (req: >= 5)

Gene RIFs: 18   (req: > 3)

Antibodies: 452   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 6

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Data Bank (13)

1 – 5 of 13

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (56)

Reactome (37)

KEGG (5)

PathwayCommons (1)

UniProt (1)

WikiPathways (12)

APC-Cdc20 mediated degradation of Nek2A (R-HSA-179409)

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Items per page:

5

1 – 5 of 37

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	APC-Cdc20 mediated degradation of Nek2A	25 targets	R-HSA-179409
Reactome	APC/C-mediated degradation of cell cycle proteins	87 targets	R-HSA-174143
Reactome	APC/C:Cdc20 mediated degradation of Cyclin B	23 targets	R-HSA-174048
Reactome	APC/C:Cdc20 mediated degradation of Securin	68 targets	R-HSA-174154
Reactome	APC/C:Cdc20 mediated degradation of mitotic proteins	76 targets	R-HSA-176409

Name	Explore in Pharos	Explore in Source
APC-Cdc20 mediated degradation of Nek2A	25 targets	R-HSA-179409
APC/C-mediated degradation of cell cycle proteins	87 targets	R-HSA-174143
APC/C:Cdc20 mediated degradation of Cyclin B	23 targets	R-HSA-174048
APC/C:Cdc20 mediated degradation of Securin	68 targets	R-HSA-174154
APC/C:Cdc20 mediated degradation of mitotic proteins	76 targets	R-HSA-176409

Gene Ontology Terms (13)

Functions (1)

Components (7)

Processes (5)

Items per page:

10

1 – 1 of 1

GO Term	Evidence	Assigned by
protein phosphatase binding	Inferred from Physical Interaction (IPI)	BHF-UCL

Protein-Protein Interactions (527)

Explore Interacting Targets

1 – 10 of 527

• See Interactions on String-DB

ANAPC16

Tdark

Family: Enzyme

Novelty: 0.09302326

p_int: 0.999999777

p_ni: 2.18e-7

p_wrong: 5e-9

Score: 0.999

Data Source: BioPlex,STRINGDB

ANAPC5

Tbio

Family: Enzyme

Novelty: 0.0345899

p_int: 0.999999549

p_ni: 4.51e-7

Score: 0.999

Data Source: BioPlex,STRINGDB

ANAPC13

Tbio

Family: Enzyme

Novelty: 0.0994907

p_int: 0.999998604

p_ni: 0.000001395

Score: 0.999

Data Source: BioPlex,STRINGDB

CDC26

Tbio

Family: Enzyme

Novelty: 0.12631579

p_int: 0.999998104

p_ni: 0.000001896

Score: 0.999

Data Source: BioPlex,STRINGDB

C16orf87

Tdark

p_int: 0.999969623

p_ni: 0.000030377

Score: 0.392

Data Source: BioPlex,STRINGDB

GRAP2

Tbio

Novelty: 0.00186531

p_int: 0.999922559

p_ni: 0.000077441

Data Source: BioPlex

MAGIX

Tdark

Novelty: 0.09090909

p_int: 0.999878297

p_ni: 0.000121698

p_wrong: 5e-9

Score: 0.173

Data Source: BioPlex,STRINGDB

TTLL1

Tdark

Family: Enzyme

Novelty: 0.14944557

p_int: 0.999741982

p_ni: 0.000257935

p_wrong: 8.3e-8

Score: 0.197

Data Source: BioPlex,STRINGDB

ANO6

Tbio

Family: IC

Novelty: 0.01570866

p_int: 0.999395078

p_ni: 0.000604922

Data Source: BioPlex

CDC16

Tbio

Novelty: 0.01336597

p_int: 0.999142349

p_ni: 0.000857646

p_wrong: 5e-9

Score: 0.999

Data Source: BioPlex,STRINGDB

Publication Statistics

PubMed Score  98.81

PubMed score by year

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PubTator Score  74.43

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQ

1-70

CKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQK

70-140

SLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTI

140-210

ELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTP

210-280

SFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQ

280-350

SSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQ

350-420

PNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSH

420-490

LPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLT

490-560

DMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRV

560-630

NPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDP

630-700

KNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKG

700-770

ANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

770-824

MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Find similar targets by:

Uniprot Keyword

3D-structure
Alternative splicing
Complete proteome
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Repeat
TPR repeat
Ubl conjugation pathway