Tbio NME2 Nucleoside diphosphate kinase B

Protein Summary

Description

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity). Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197). Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752, PubMed:19435876). Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1) (PubMed:19435876). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041). Exhibits histidine protein kinase activity (PubMed:20946858). Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

Uniprot Accession IDs

• P22392
• A8MWA3
• Q1WM23
• Q6LCT6
• NDK B

Gene Name

• NME2

Ensembl ID

• ENST00000393190
• ENSP00000376886
• ENSG00000243678
• ENST00000503064
• ENSP00000426901
• ENST00000512737
• ENSP00000421064
• ENST00000513177
• ENSP00000425581
• ENST00000514264
• ENSP00000426976

Symbol

• NM23B
• PUF
• NDKB
• NDPKB
• NM23B
• NDPK-B
• NM23-H2

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
cell type or tissue	0.72
histone modification site profile	0.71
small molecule perturbation	0.71
cellular component	0.63
disease	0.63

Protein Classes

DTO Classes

Protein / Kinase / Unclassified Protein / Nucleoside Diphosphate Kinase B

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 162.93   (req: < 5)

Gene RIFs: 51   (req: <= 3)

Antibodies: 90   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 162.93   (req: >= 5)

Gene RIFs: 51   (req: > 3)

Antibodies: 90   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 37

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0