Tbio PFKL ATP-dependent 6-phosphofructokinase, liver type

Protein Classes

PANTHER Classes

Protein / Transferase / Kinase / Carbohydrate Kinase / Atp-dependent 6-Phosphofructokinase, Liver Type

DTO Classes

Protein / Kinase / Non-protein Kinase / Carbohydrate Kinase / Phosphofructokinase Type A (Pfka) Family / Atp-dependent Pfk Group I Subfamily / Eukaryotic Two Domain Clade "E" Sub-subfamily / Atp-dependent 6-Phosphofructokinase, Liver Type

Protein Summary

Description

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity). This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Accession IDs

• P17858
• Q96A64
• Q96IH4
• Q9BR91
• ATP-PFK

Gene Name

• PFKL

Ensembl ID

• ENST00000349048
• ENSP00000269848
• ENSG00000141959

Symbol

• PFK-B
• PFK-L
• ATP-PFK

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
molecular function	0.99
disease perturbation	0.87
transcription factor perturbation	0.84
interacting protein	0.76
hub protein	0.75

Related Tools

Target Illumination GWAS Analytics (TIGA)

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GENEVA

GENEVA (GENe Expression Variance Analysis) allows you to identify RNA-sequencing datasets from the Gene Expression Omnibus (GEO) that contain conditions modulating a gene or a gene signature.

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GlyGen

GlyGen is a data integration and dissemination project for carbohydrate and glycoconjugate related data.

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ARCHS4

ARCHS4 provides access to gene-function predictions based on RNA-seq co-expression, and gene expression levels across cell and tissues.

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IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 157.01   (req: < 5)

Gene RIFs: 6   (req: <= 3)

Antibodies: 301   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 157.01   (req: >= 5)

Gene RIFs: 6   (req: > 3)

Antibodies: 301   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 19

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

GWAS Traits (2)

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GWAS Trait	EFO ID	Study Count	SNP Count	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
hematocrit	EFO_0004348	1	1	1		27
hemoglobin measurement	EFO_0004509	1	1	1		19.4

• Explore on Target Illumination GWAS Analytics (TIGA)

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GWAS Trait	EFO ID	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
hematocrit	EFO_0004348	1		27
hemoglobin measurement	EFO_0004509	1		19.4

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Orthologs (12)

1 – 5 of 12

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	phosphofructokinase, liver type	VGNC:7549	458602
Mouse	phosphofructokinase, liver, B-type	MGI:97547	18641
Rat	phosphofructokinase, liver type	RGD:3311	25741
Dog	phosphofructokinase, liver type	VGNC:44446	487797
Horse	phosphofructokinase, liver type	VGNC:21341	100056927

Species	Name	OMA	EggNOG	Inparanoid
Chimp	phosphofructokinase, liver type
Mouse	phosphofructokinase, liver, B-type
Rat	phosphofructokinase, liver type
Dog	phosphofructokinase, liver type
Horse	phosphofructokinase, liver type

Protein Structure (0 Structures, 1 AlphaFold Model)

Representation

  axes   backbone   ball+stick   cartoon   rocket   rope   spacefill

Color Scheme

  target   atomindex   bfactor   chainname   electrostatic   element   hydrophobicity   resname   sstruc

AF-P17858-F1-model_v1

AlphaFold Structures Developed by DeepMind and EMBL-EBI

Pathways (30)

Reactome (7)

KEGG (12)

PathwayCommons (5)

UniProt (1)

WikiPathways (5)

Glucose metabolism (R-HSA-70326)

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Find Similar Targets

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Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Glucose metabolism	92 targets	R-HSA-70326
Reactome	Glycolysis	72 targets	R-HSA-70171
Reactome	Immune System	2246 targets	R-HSA-168256
Reactome	Innate Immune System	1188 targets	R-HSA-168249
Reactome	Metabolism	2112 targets	R-HSA-1430728

Name	Explore in Pharos	Explore in Source
Glucose metabolism	92 targets	R-HSA-70326
Glycolysis	72 targets	R-HSA-70171
Immune System	2246 targets	R-HSA-168256
Innate Immune System	1188 targets	R-HSA-168249
Metabolism	2112 targets	R-HSA-1430728

Gene Ontology Terms (26)

Functions (9)

Components (7)

Processes (10)

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1 – 9 of 9

GO Term	Evidence	Assigned by
6-phosphofructokinase activity	Inferred from Direct Assay (IDA)	UniProtKB
ATP binding	Inferred from Direct Assay (IDA)	UniProtKB
fructose binding	Inferred from Direct Assay (IDA)	BHF-UCL
fructose-6-phosphate binding	Inferred from Direct Assay (IDA)	BHF-UCL
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
kinase binding	Inferred from Physical Interaction (IPI)	BHF-UCL
AMP binding	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
monosaccharide binding	Inferred from Biological aspect of Ancestor (IBA)	GO_Central
metal ion binding	Inferred from Electronic Annotation (IEA)	UniProtKB-KW

Protein-Protein Interactions (135)

Explore Interacting Targets

1 – 10 of 135

• See Interactions on String-DB

PFKP

Tbio

Family: Kinase

Novelty: 0.00808684

p_int: 0.99900033

p_ni: 0.00099967

Score: 0.987

Data Source: BioPlex,Reactome,STRINGDB

TFR2

Tbio

Family: Enzyme

Novelty: 0.00480264

p_int: 0.762626566

p_ni: 0.237373434

Score: 0.365

Data Source: BioPlex,STRINGDB

PFKM

Tbio

Family: Kinase

Novelty: 0.00217816

Score: 0.994

Data Source: Reactome,STRINGDB

GPI

Tbio

Family: Enzyme

Novelty: 0.00095404

Score: 0.991

Data Source: STRINGDB

ALDOA

Tbio

Family: Enzyme

Novelty: 0.00517474

Score: 0.978

Data Source: STRINGDB

ALDOC

Tbio

Family: Enzyme

Novelty: 0.01552954

Score: 0.977

Data Source: STRINGDB

ALDOB

Tbio

Family: Enzyme

Novelty: 0.00856695

Score: 0.976

Data Source: STRINGDB

TALDO1

Tbio

Family: Enzyme

Novelty: 0.00208619

Score: 0.961

Data Source: STRINGDB

TKT

Tchem

Family: Enzyme

Novelty: 0.00333936

Score: 0.949

Data Source: STRINGDB

FBP2

Tbio

Family: Enzyme

Novelty: 0.00854851

Score: 0.948

Data Source: STRINGDB

Publication Statistics

PubMed Score  157.01

PubMed score by year

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PubTator Score  47.34

PubTator score by year

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Patents

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Amino Acid Sequence

Residue Counts

Protein Sequence

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UniProt Keyword Find Similar Targets

Acetylation
Allosteric enzyme
Alternative splicing
ATP-binding
Complete proteome
Cytoplasm
Direct protein sequencing
Glycolysis
Glycoprotein
Kinase

GWAS Trait Find Similar Targets

hematocrit
hemoglobin measurement

MGI Phenotype Find Similar Targets

homeostasis/metabolism phenotype
integument phenotype
mortality/aging