Tbio XRCC5 X-ray repair cross-complementing protein 5

Protein Summary

Description

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P13010
• A8K3X5
• Q0Z7V0
• Q4VBQ5
• Q53HH7
• Q7M4N0
• Q9UCQ0
• Q9UCQ1

Gene Name

• XRCC5

Ensembl ID

• ENST00000392132
• ENSP00000375977
• ENSG00000079246
• ENST00000392133
• ENSP00000375978

Symbol

• G22P2
• KU80
• KUB2
• Ku86
• NFIV
• KARP1
• KARP-1

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
molecular function	1
co-expressed gene	0.99
transcription factor perturbation	0.99
virus perturbation	0.99
hub protein	0.98

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Helicase / Dna Helicase / X-ray Repair Cross-complementing Protein 5

Protein / Nucleic Acid Binding / Dna Binding Protein / Dna Helicase / X-ray Repair Cross-complementing Protein 5

DTO Classes

Protein / Enzyme / Helicase / Dna Helicase / X-ray Repair Cross-complementing Protein 5

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 715.77   (req: < 5)

Gene RIFs: 180   (req: <= 3)

Antibodies: 1075   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 715.77   (req: >= 5)

Gene RIFs: 180   (req: > 3)

Antibodies: 1075   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 41

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0