Tchem GAPDH Glyceraldehyde-3-phosphate dehydrogenase

Protein Classes

PANTHER Classes

Protein / Oxidoreductase / Dehydrogenase / Glyceraldehyde-3-phosphate Dehydrogenase

DTO Classes

Protein / Enzyme / Oxidoreductase / Dehydrogenase / Glyceraldehyde-3-phosphate Dehydrogenase

Protein Summary

Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Accession IDs

• P04406
• E7EUT4
• P00354
• Q53X65
• GAPDH

Gene Name

• GAPDH

Ensembl ID

• ENST00000229239
• ENSP00000229239
• ENSG00000111640
• ENST00000396858
• ENSP00000380067
• ENST00000396859
• ENSP00000380068
• ENST00000396861
• ENSP00000380070
• ENST00000619601
• ENSP00000478864

Symbol

• GAPD
• G3PD
• GAPD
• HEL-S-162eP

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
disease perturbation	1
hub protein	1
interacting protein	1
chemical	0.96
kinase perturbation	0.95

IDG Partner Tools

GlyGen

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 11242.06   (req: < 5)

Gene RIFs: 131   (req: <= 3)

Antibodies: 2468   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 11242.06   (req: >= 5)

Gene RIFs: 131   (req: > 3)

Antibodies: 2468   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 25

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 5

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Active Ligands (5)

Explore Active Ligands

1 – 5 of 5

CHEMBL3350919

GAPDHTchem Enzyme

IC50: 7.22

CHEMBL124807

GAPDHTchem Enzyme

IC50: 6.3

CHEMBL256155

GAPDHTchem Enzyme

IC50: 6.23

CHEMBL3142964

GAPDHTchem Enzyme

IC50: 6.52

CHEMBL3559427

GAPDHTchem Enzyme

IC50: 7

Protein Data Bank (7)

1 – 5 of 7

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (27)

Reactome (5)

KEGG (6)

PathwayCommons (8)

UniProt (1)

WikiPathways (7)

Gluconeogenesis (R-HSA-70263)

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Items per page:

5

1 – 5 of 5

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Gluconeogenesis	34 targets	R-HSA-70263
Reactome	Glucose metabolism	92 targets	R-HSA-70326
Reactome	Glycolysis	72 targets	R-HSA-70171
Reactome	Metabolism	2112 targets	R-HSA-1430728
Reactome	Metabolism of carbohydrates	293 targets	R-HSA-71387

Name	Explore in Pharos	Explore in Source
Gluconeogenesis	34 targets	R-HSA-70263
Glucose metabolism	92 targets	R-HSA-70326
Glycolysis	72 targets	R-HSA-70171
Metabolism	2112 targets	R-HSA-1430728
Metabolism of carbohydrates	293 targets	R-HSA-71387

Gene Ontology Terms (39)

Functions (8)

Components (14)

Processes (17)

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Items per page:

10

1 – 8 of 8

GO Term	Evidence	Assigned by
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity	Inferred from Experiment (EXP)	Reactome
aspartic-type endopeptidase inhibitor activity	Inferred from Direct Assay (IDA)	UniProtKB
disordered domain specific binding	Inferred from Physical Interaction (IPI)	CAFA
identical protein binding	Inferred from Physical Interaction (IPI)	IntAct
microtubule binding	Inferred from Sequence or structural Similarity (ISS)	UniProtKB
peptidyl-cysteine S-nitrosylase activity	Inferred from Sequence or structural Similarity (ISS)	UniProtKB
NAD binding	Inferred from Electronic Annotation (IEA)	InterPro
NADP binding	Inferred from Electronic Annotation (IEA)	InterPro

Protein-Protein Interactions (1596)

Explore Interacting Targets

1 – 10 of 1596

• See Interactions on String-DB

GAPDHS

Tbio

Family: Enzyme

Novelty: 0.00885959

p_int: 0.999999808

p_ni: 1.75e-7

p_wrong: 1.7e-8

Score: 0.844

Data Source: BioPlex,STRINGDB

ASPSCR1

Tbio

Novelty: 0.01172319

p_int: 0.993715189

p_ni: 0.006284811

Score: 0.595

Data Source: BioPlex,STRINGDB

RABGGTA

Tdark

Family: Enzyme

Novelty: 0.19962321

p_int: 0.913290897

p_ni: 0.086709103

Score: 0.211

Data Source: BioPlex,STRINGDB

ZIC1

Tbio

Family: TF

Novelty: 0.00491601

p_int: 0.769814755

p_ni: 0.230185245

Score: 0.378

Data Source: BioPlex,STRINGDB

TPI1

Tbio

Family: Enzyme

Novelty: 0.00074652

Score: 0.999

Data Source: STRINGDB

PGK1

Tchem

Family: Kinase

Novelty: 0.00113494

Score: 0.998

Data Source: STRINGDB

ALDOA

Tbio

Family: Enzyme

Novelty: 0.00517474

Score: 0.995

Data Source: STRINGDB

ENO1

Tchem

Family: Enzyme

Novelty: 0.00109178

Score: 0.995

Data Source: STRINGDB

RPL13A

Tbio

Novelty: 0.00503698

Score: 0.99

Data Source: STRINGDB

GPI

Tbio

Family: Enzyme

Novelty: 0.00095404

Score: 0.986

Data Source: STRINGDB

Publication Statistics

PubMed Score  11242.06

PubMed score by year

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PubTator Score  2556.29

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Amino Acid Sequence

Residue Counts

Protein Sequence

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3D-structure
Acetylation
ADP-ribosylation
Alternative splicing
Apoptosis
Complete proteome
Cytoplasm
Cytoskeleton
Direct protein sequencing
Glycolysis

MGI Phenotype Find Similar Targets

hematopoietic system phenotype
homeostasis/metabolism phenotype
mortality/aging