Tbio TOP3B DNA topoisomerase 3-beta-1

Protein Summary

Description

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]

Uniprot Accession IDs

• O95985
• A0M8Q3
• Q9BUP5

Gene Name

• TOP3B

Ensembl ID

• ENST00000357179
• ENSP00000349705
• ENSG00000100038
• ENST00000398793
• ENSP00000381773

Symbol

• TOP3B1
• TOP3B1

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
protein domain	0.99
transcription factor binding site profile	0.88
disease perturbation	0.79
cell line	0.63
transcription factor	0.63

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Dna Topoisomerase 3-beta-1

Protein / Isomerase / Dna Topoisomerase 3-beta-1

Protein / Enzyme Modulator / Dna Topoisomerase 3-beta-1

DTO Classes

Protein / Enzyme / Isomerase / Dna Topoisomerase 3-beta-1

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 21.78   (req: < 5)

Gene RIFs: 16   (req: <= 3)

Antibodies: 191   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 21.78   (req: >= 5)

Gene RIFs: 16   (req: > 3)

Antibodies: 191   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 6

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0