Tchem MGAT3 Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Protein Summary

Description

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains, called bisecting N-acetylglucosamine (GlcNAc). It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The addition of this bisecting GlcNAc residue alters not only the composition, but also the conformation of the N-glycan. The introduction of the bisecting GlcNAc residue results in the suppression of further processing and elongation of N-glycans, precluding the formation of beta-1,6 GlcNAc branching, catalyzed by MGAT5 since it is unable to use the bisected oligosaccharide as a substrate (PubMed:19403558). Addition of bisecting N-acetylglucosamine to CDH1/E-cadherin modulates CDH1 cell membrane location (PubMed:19403558). Inhibits NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc- formation which modulates sialylation levels and plays a role in cell migration regulation (PubMed:26801611). In brain, addition of bisecting N-acetylglucosamine to BACE1 blocks its lysosomal targeting in response to oxidative stress and further degradation which increases its location to early endosome and the APP cleavage (By similarity). There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• Q09327
• A6NGD0
• Q14CK5
• Q6IC49
• Q9UH32

Gene Name

• MGAT3

Ensembl ID

• ENST00000341184
• ENSP00000345270
• ENSG00000128268

Symbol

• GGNT3
• GNT3
• GNT-III

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
PubMedID	0.71
tissue sample	0.7
virus perturbation	0.68
cellular component	0.65
transcription factor	0.65

Protein Classes

PANTHER Classes

Protein / Transferase / Glycosyltransferase / Beta-1,4-mannosyl-glycoprotein 4-beta-n-acetylglucosaminyltransferase

DTO Classes

Protein / Enzyme / Transferase / Glycosyltransferase / Beta-1,4-mannosyl-glycoprotein 4-beta-n-acetylglucosaminyltransferase

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 27.17   (req: < 5)

Gene RIFs: 23   (req: <= 3)

Antibodies: 217   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 27.17   (req: >= 5)

Gene RIFs: 23   (req: > 3)

Antibodies: 217   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 11

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 11

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0