Tchem GPR183 G-protein coupled receptor 183

Protein Summary

Description

G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity). Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (PubMed:21796212, PubMed:22875855, PubMed:22930711). Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity). Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity). Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity). Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (PubMed:22913878). Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity). Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity). Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels (By similarity). Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (PubMed:25297897). Promotes osteoclast precursor migration to bone surfaces (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha (PubMed:21673108, PubMed:25297897). Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity). This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]

Uniprot Accession IDs

• P32249
• B2R8N5
• Q53F99
• Q5JUH7

Gene Name

• GPR183

Ensembl ID

• ENST00000376414
• ENSP00000365596
• ENSG00000169508

Symbol

• EBI2
• EBI2
• hEBI2

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
gene perturbation	0.94
PubMedID	0.78
tissue sample	0.67
tissue	0.64
biological process	0.6

Protein Classes

DTO Classes

Protein / G-protein Coupled Receptor / Class A Rhodopsin Like / G-protein Coupled Receptor 183

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 69.09   (req: < 5)

Gene RIFs: 15   (req: <= 3)

Antibodies: 194   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 69.09   (req: >= 5)

Gene RIFs: 15   (req: > 3)

Antibodies: 194   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 16

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 87

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Protein Sequence and Structure

Residue Counts

Protein Sequence

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Expression Data (0 Tissues)

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No expression data found

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Target Illumination GWAS Analytics (TIGA)

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GENEVA

GENEVA (GENe Expression Variance Analysis) allows you to identify RNA-sequencing datasets from the Gene Expression Omnibus (GEO) that contain conditions modulating a gene or a gene signature.

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GlyGen

GlyGen is a data integration and dissemination project for carbohydrate and glycoconjugate related data.

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ARCHS4

ARCHS4 provides access to gene-function predictions based on RNA-seq co-expression, and gene expression levels across cell and tissues.

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Approved Drugs (0)

No approved drugs found

Active Ligands (87)

Explore Active Ligands

1 – 10 of 87

25-hydroxycholesterol

GPR183Tchem GPCR

pEC50 : 7.7

27-hydroxycholesterol

GPR183Tchem GPCR

: 7.15

7alpha-hydroxycholesterol

GPR183Tchem GPCR

pKi : 8

7beta-hydroxycholesterol

GPR183Tchem GPCR

pKi : 8

7&alpha;,27-dihydroxycholesterol

GPR183Tchem GPCR

pEC50 : 8.89

7&beta;, 25-dihydroxycholesterol

GPR183Tchem GPCR

pEC50 : 8.68

7&beta;, 27-dihydroxycholesterol

GPR183Tchem GPCR

pEC50 : 7.29

SID174315875

GPR183Tchem GPCR

pIC50 : 9.65

SID174315901

GPR183Tchem GPCR

pIC50 : 9.39

SID174315860

GPR183Tchem GPCR

pIC50 : 9.21

Protein-Protein Interactions (296)

Explore Interacting Targets

1 – 10 of 296

• See Interactions on String-DB

Q9HDB5

Tbio

Novelty:  0.00999084

p_int:  0.999999686

p_ni:  3.11e-7

p_wrong:  2e-9

Score:  0.187

Data Source:  BioPlex,STRINGDB

SDC2

Tbio

Novelty:  0.00597758

p_int:  0.999999681

p_ni:  3.18e-7

p_wrong:  1e-9

Data Source:  BioPlex

GPC1

Tbio

Novelty:  0.00293087

p_int:  0.999999528

p_ni:  4.72e-7

Score:  0.187

Data Source:  BioPlex,STRINGDB

TSHZ3

Tbio

Family:  TF

Novelty:  0.02963612

p_int:  0.999997153

p_ni:  0.000002817

p_wrong:  3.1e-8

Score:  0.161

Data Source:  BioPlex,STRINGDB

TMEFF1

Tbio

Novelty:  0.11856207

p_int:  0.999992146

p_ni:  1.22e-7

p_wrong:  0.000007732

Data Source:  BioPlex

TMED8

Tdark

Novelty:  1.41977071

p_int:  0.999986342

p_ni:  0.000003781

p_wrong:  0.000009877

Score:  0.181

Data Source:  BioPlex,STRINGDB

STAG2

Tbio

Novelty:  0.00430441

p_int:  0.999970769

p_ni:  0.000029231

Data Source:  BioPlex

EIF2B1

Tbio

Novelty:  0.02520554

p_int:  0.999909886

p_ni:  0.00009009

p_wrong:  2.4e-8

Score:  0.518

Data Source:  BioPlex,STRINGDB

TGFBR3

Tbio

Novelty:  0.0077307

p_int:  0.999883983

p_ni:  0.000116017

Data Source:  BioPlex

EIF2B5

Tbio

Novelty:  0.0102209

p_int:  0.999858056

p_ni:  0.000141944

Score:  0.264

Data Source:  BioPlex,STRINGDB

Nearest Tclin Targets

Nearest Tclin calculations are only available for targets with KEGG Pathway annotations.

Pathways (8)

Reactome (6)

WikiPathways (2)

Class A/1 (Rhodopsin-like receptors) (R-HSA-373076)

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Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Class A/1 (Rhodopsin-like receptors)	323 targets	R-HSA-373076
Reactome	G alpha (i) signalling events	408 targets	R-HSA-418594
Reactome	GPCR downstream signalling	1152 targets	R-HSA-388396
Reactome	GPCR ligand binding	460 targets	R-HSA-500792
Reactome	Signal Transduction	2704 targets	R-HSA-162582

Name	Explore in Pharos	Explore in Source
Class A/1 (Rhodopsin-like receptors)	323 targets	R-HSA-373076
G alpha (i) signalling events	408 targets	R-HSA-418594
GPCR downstream signalling	1152 targets	R-HSA-388396
GPCR ligand binding	460 targets	R-HSA-500792
Signal Transduction	2704 targets	R-HSA-162582

Viral Interactions (0)

No viral interactions found

Gene Ontology Terms (18)

Functions (2)

Components (2)

Processes (14)

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1 – 2 of 2

GO Term	Evidence	Assigned by
G protein-coupled receptor activity	Inferred from Direct Assay (IDA)	UniProtKB
oxysterol binding	Inferred from Direct Assay (IDA)	UniProtKB

Disease Associations ()

No disease associations found

GWAS Traits (12)

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GWAS Trait	EFO ID	Study Count	SNP Count	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
eosinophil count	EFO_0004842	3	3	3		94.4
eosinophil percentage of leukocytes	EFO_0007991	2	2	2		89.6
asthma	EFO_0000270	4	4	0	1.1	88.3
inflammatory bowel disease	EFO_0003767	2	2	0	1.1	73.4
childhood onset asthma	EFO_0004591	2	1	0	1.1	72.3

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GWAS Trait	EFO ID	Beta Count	Odds Ratio	Evidence (Mean Rank Score)	Provenance
eosinophil count	EFO_0004842	3		94.4
eosinophil percentage of leukocytes	EFO_0007991	2		89.6
asthma	EFO_0000270	0	1.1	88.3
inflammatory bowel disease	EFO_0003767	0	1.1	73.4
childhood onset asthma	EFO_0004591	0	1.1	72.3

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UniProt Keyword Find Similar Targets

No Header	No Header
Adaptive immunity
Cell membrane
Complete proteome
Disulfide bond
G-protein coupled receptor
Immunity
Membrane
Phosphoprotein
Polymorphism
Receptor

GWAS Trait Find Similar Targets

No Header	No Header
asthma
basal cell carcinoma
childhood onset asthma
Crohn's disease
eosinophil count
eosinophil percentage of granulocytes
eosinophil percentage of leukocytes
inflammatory bowel disease
Inhalant adrenergic use measurement
keratinocyte carcinoma

MGI Phenotype Find Similar Targets

No Header	No Header
hematopoietic system phenotype
homeostasis/metabolism phenotype
immune system phenotype

IDG Resources

Reagents (1)

Genetic Construct

GPR183-Tango

Details:

• Vector:  addgene66342
• Vector backbone:  pcDNA3.1(+)
• Promoter:  CMV
• Tag:  FLAG

Data:

• GPR183-Tango

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addgene

Orthologs (15)

1 – 5 of 15

Species	Name	Source ID	Gene ID	OMA	EggNOG	Inparanoid
Chimp	G protein-coupled receptor 183	VGNC:8877	467308
Macaque	G protein-coupled receptor 183		698844
Mouse	G protein-coupled receptor 183	MGI:2442034	321019
Rat	G protein-coupled receptor 183	RGD:1598095	679975
Dog	G protein-coupled receptor 183	VGNC:41423	102154241

Species	Name	OMA	EggNOG	Inparanoid
Chimp	G protein-coupled receptor 183
Macaque	G protein-coupled receptor 183
Mouse	G protein-coupled receptor 183
Rat	G protein-coupled receptor 183
Dog	G protein-coupled receptor 183

Publication Statistics

PubMed Score 69.09

PubMed score by year

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PubTator Score 33.58

PubTator score by year

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Patents

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Related Publications

Text Mined References (23)

GeneRif Annotations (15)

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PMID	Year	Title