Tchem MMP13 Collagenase 3

Protein Classes

PANTHER Classes

Protein / ... / Metalloprotease / Collagenase 3

DTO Classes

Protein / Enzyme / Protease / Metalloprotease / Collagenase 3

Protein Summary

Description

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Uniprot Accession IDs

• P45452
• A8K846
• B2RCZ3
• Q6NWN6

Gene Name

• MMP13

Ensembl ID

• ENST00000260302
• ENSP00000260302
• ENSG00000137745

Symbol

• CLG3
• MDST
• MANDP1
• MMP-13

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
protein domain	1
biological term	0.98
gene perturbation	0.85
chemical	0.82
disease	0.76

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 1361.96   (req: < 5)

Gene RIFs: 259   (req: <= 3)

Antibodies: 971   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 1361.96   (req: >= 5)

Gene RIFs: 259   (req: > 3)

Antibodies: 971   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 13

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 2045

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0

Active Ligands (2045)

Explore Active Ligands

1 – 10 of 2045

CHEMBL514138

MMP13Tchem Enzyme

Ki: 8.9

CHEMBL19611

MMP13Tchem Enzyme

IC50: 8.28

CHEMBL279785

MMP13Tchem Enzyme

IC50: 8.92

CHEMBL440498

MMP13Tchem Enzyme

Ki: 9.55

CHEMBL75094

MMP13Tchem Enzyme

Ki: 10.42

CHEMBL279786

MMP13Tchem Enzyme

IC50: 8.3

CHEMBL115653

MMP13Tchem Enzyme

Ki: 9.28

CHEMBL70176

MMP13Tchem Enzyme

IC50: 8.1

CHEMBL187092

MMP13Tchem Enzyme

IC50: 9.05

CHEMBL3675563

MMP13Tchem Enzyme

Ki: 7.7

Protein Data Bank (48)

1 – 5 of 48

PDB Structure Id	Ligand	Method	Resolution (Å)	M.W. (kDa)	Pub Year	Title

PDB Structure Id	M.W.	Resolution	Pub Year

Pathways (68)

Reactome (11)

KEGG (3)

PathwayCommons (3)

WikiPathways (51)

Activation of Matrix Metalloproteinases (R-HSA-1592389)

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Items per page:

5

1 – 5 of 11

Data Source	Name	Explore in Pharos	Explore in Source
Reactome	Activation of Matrix Metalloproteinases	33 targets	R-HSA-1592389
Reactome	Assembly of collagen fibrils and other multimeric structures	57 targets	R-HSA-2022090
Reactome	Collagen degradation	40 targets	R-HSA-1442490
Reactome	Collagen formation	90 targets	R-HSA-1474290
Reactome	Degradation of the extracellular matrix	111 targets	R-HSA-1474228

Name	Explore in Pharos	Explore in Source
Activation of Matrix Metalloproteinases	33 targets	R-HSA-1592389
Assembly of collagen fibrils and other multimeric structures	57 targets	R-HSA-2022090
Collagen degradation	40 targets	R-HSA-1442490
Collagen formation	90 targets	R-HSA-1474290
Degradation of the extracellular matrix	111 targets	R-HSA-1474228

Gene Ontology Terms (16)

Functions (5)

Components (3)

Processes (8)

Items per page:

10

1 – 5 of 5

GO Term	Evidence	Assigned by
calcium ion binding	Inferred from Direct Assay (IDA)	UniProtKB
collagen binding	Inferred from Direct Assay (IDA)	UniProtKB
metalloendopeptidase activity	Inferred from Direct Assay (IDA)	UniProtKB
zinc ion binding	Inferred from Direct Assay (IDA)	UniProtKB
serine-type endopeptidase activity	Traceable Author Statement (TAS)	Reactome

Protein-Protein Interactions (199)

Explore Interacting Targets

1 – 10 of 199

• See Interactions on String-DB

MYCBP2

Tbio

Family: Enzyme

Novelty: 0.00336142

p_int: 0.999985544

p_ni: 0.000014456

Data Source: BioPlex

FBXO45

Tbio

Novelty: 0.08978487

p_int: 0.999706516

p_ni: 0.000283778

p_wrong: 0.000009706

Data Source: BioPlex

PEAK1

Tbio

Family: Kinase

Novelty: 0.00254215

p_int: 0.997177393

p_ni: 0.002822606

p_wrong: 1e-9

Data Source: BioPlex

SPRYD3

Tdark

p_int: 0.991992985

p_ni: 0.000021102

p_wrong: 0.007985913

Data Source: BioPlex

HTRA2

Tbio

Family: Enzyme

Novelty: 0.00078515

p_int: 0.991298641

p_ni: 0.008701359

Data Source: BioPlex

GID4

Tbio

Novelty: 0.10469899

p_int: 0.983636686

p_ni: 0.016363314

Data Source: BioPlex

ARMC8

Tbio

Novelty: 0.08450354

p_int: 0.982847074

p_ni: 0.017152926

Data Source: BioPlex

WDR26

Tbio

Novelty: 0.07848267

p_int: 0.982235842

p_ni: 0.017764159

Data Source: BioPlex

ADAMTS2

Tbio

Family: Enzyme

Novelty: 0.00060547

p_int: 0.945194648

p_ni: 0.054542912

p_wrong: 0.00026244

Score: 0.362

Data Source: BioPlex,STRINGDB

MKLN1

Tbio

Novelty: 0.07307009

p_int: 0.944621862

p_ni: 0.055378138

Data Source: BioPlex

Publication Statistics

PubMed Score  1361.96

PubMed score by year

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PubTator Score  1057.39

PubTator score by year

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Amino Acid Sequence

Residue Counts

Sequence

MHPGVLAAFLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENAASSMTERLRE

1-70

MQSFFGLEVTGKLDDNTLDVMKKPRCGVPDVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKK

70-140

AFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSS

140-210

SKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKT

210-280

PDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFR

280-350

GRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLI

350-420

EEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC

420-471

MHPGVLAAFLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENAASSMTERLREMQSFFGLEVTGKLDDNTLDVMKKPRCGVPDVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC

Find similar targets by:

Uniprot Keyword

3D-structure
Calcium
Collagen degradation
Complete proteome
Direct protein sequencing
Disease mutation
Disulfide bond
Dwarfism
Extracellular matrix
Glycoprotein