| Tbio | Charged multivesicular body protein 3 |
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress-mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.
This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010]
This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010]
Comments
| Disease | Target Count | P-value |
|---|---|---|
| lung adenocarcinoma | 2714 | 8.48199824128774E-16 |
| ovarian cancer | 8492 | 6.63713611180552E-9 |
| osteosarcoma | 7933 | 1.88765009072355E-7 |
| atypical teratoid / rhabdoid tumor | 4369 | 1.6966363617931E-6 |
| medulloblastoma, large-cell | 6234 | 8.37756035286666E-5 |
| lung cancer | 4473 | 0.00254741325251909 |
| Multiple myeloma | 1328 | 0.00612262645661606 |
| Breast cancer | 3099 | 0.0432884395263116 |
| Disease | Target Count | Z-score | Confidence |
|---|---|---|---|
| Brain glioma | 10 | 3.111 | 1.6 |
| Disease | log2 FC | p |
|---|---|---|
| Multiple myeloma | 1.007 | 0.006 |
| osteosarcoma | 2.180 | 0.000 |
| atypical teratoid / rhabdoid tumor | -1.300 | 0.000 |
| medulloblastoma, large-cell | -1.200 | 0.000 |
| lung cancer | -1.200 | 0.003 |
| Breast cancer | 2.700 | 0.043 |
| lung adenocarcinoma | -1.600 | 0.000 |
| ovarian cancer | -2.000 | 0.000 |
| PMID | Text |
|---|---|
| 24440309 | Protein kinase CK2 alpha is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly. |
| 23051622 | Recruitment of CHMP2A and CHMP3 by CHMP4B to HIV-1 Gag puncta is observed in in-vitro membrane model |
| 23027949 | Recruitment of CHMP2A and CHMP3 by CHMP4B to HIV-1 Gag puncta is observed in in-vitro membrane model |
| 21827950 | tight coupling of ESCRT-III CHMP3 and AMSH functions and provide insight into the regulation of ESCRT-III |
| 20427536 | Recruitment of CHMP2A and CHMP3 by CHMP4B to HIV-1 Gag puncta is observed in in-vitro membrane model |
| 19525971 | Data show that the N-terminal core domains of increased sodium tolerance-1 (IST1) and charged multivesicular body protein-3 (CHMP3) form equivalent four-helix bundles, revealing that IST1 is a previously unrecognized ESCRT-III family member. |
| 18687924 | study found the ESCRT-III proteins CHMP2A & CHMP3 could assemble in vitro into helical tubular structures that expose their membrane interaction sites on the outside of the tubule; VPS4 could bind on the inside of the tubule & disassemble the tubes |
| 17711858 | UBPY MIT domain and another ubiquitin isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7. |
| 17686835 | expression of dominant negative forms of Vps4 and Vps24, two components of the MVB pathway, rresult in an impairment in infectious herpes simplex virus assembly/egress |
| 17331679 | Data demonstrate that the VPS24 gene gives rise to two functionally distinct proteins, one of which is involved in the ESCRT pathway and another novel protein that serves an anti-apoptotic role. |
| More... |
| PMID | Year | Title |
|---|---|---|
| 26496610 | 2015 | A human interactome in three quantitative dimensions organized by stoichiometries and abundances. |
| 25416956 | 2014 | A proteome-scale map of the human interactome network. |
| 24878737 | 2014 | Structure of cellular ESCRT-III spirals and their relationship to HIV budding. |
| 24440309 | 2014 | CK2 involvement in ESCRT-III complex phosphorylation. |
| 23376485 | 2013 | Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. |
| 23186163 | 2013 | Toward a comprehensive characterization of a human cancer cell phosphoproteome. |
| 23105106 | 2012 | Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport. |
| 23051622 | 2013 | ESCRT-III CHMP2A and CHMP3 form variable helical polymers in vitro and act synergistically during HIV-1 budding. |
| 22660413 | 2012 | Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. |
| 21827950 | 2011 | Structural basis for ESCRT-III CHMP3 recruitment of AMSH. |
| More... |
