Tchem CPSF3 Cleavage and polyadenylation specificity factor subunit 3

Protein Summary

Description

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]

Uniprot Accession IDs

• Q9UKF6
• O14769
• Q53RS2
• Q96F36

Gene Name

• CPSF3

Ensembl ID

• ENST00000238112
• ENSP00000238112
• ENSG00000119203

Symbol

• CPSF73
• CPSF73
• CPSF-73

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
disease perturbation	0.98
interacting protein	0.89
kinase perturbation	0.88
histone modification site profile	0.87
transcription factor perturbation	0.74

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Nuclease / Endoribonuclease / Cleavage And Polyadenylation Specificity Factor Subunit 3

Protein / Nucleic Acid Binding / Rna Binding Protein / Mrna Processing Factor / Mrna Polyadenylation Factor / Cleavage And Polyadenylation Specificity Factor Subunit 3

Protein / Nucleic Acid Binding / Rna Binding Protein / Endoribonuclease / Cleavage And Polyadenylation Specificity Factor Subunit 3

DTO Classes

Protein / Enzyme / Nuclease / Endoribonuclease / Cleavage And Polyadenylation Specificity Factor Subunit 3

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 77.24   (req: < 5)

Gene RIFs: 17   (req: <= 3)

Antibodies: 218   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 77.24   (req: >= 5)

Gene RIFs: 17   (req: > 3)

Antibodies: 218   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 14

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 1

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0