| Tbio | Protein argonaute-3 |
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Comments
Property Summary
| NCBI Gene PubMed Count | 24 |
| Grant Count | 10 |
| R01 Count | 6 |
| Funding | $1,138,031 |
| PubMed Score | 12.70 |
| PubTator Score | 16.63 |
| Disease | log2 FC | p |
|---|---|---|
| Rheumatoid Arthritis | 1.800 | 0.014 |
| pancreatic cancer | 1.100 | 0.001 |
| astrocytic glioma | 1.700 | 0.013 |
| osteosarcoma | 1.510 | 0.000 |
| atypical teratoid / rhabdoid tumor | 1.600 | 0.001 |
| glioblastoma | -1.200 | 0.003 |
| medulloblastoma, large-cell | -1.400 | 0.000 |
| tuberculosis and treatment for 6 months | -1.100 | 0.017 |
| pancreatic carcinoma | 1.100 | 0.001 |
| psoriasis | -1.100 | 0.000 |
| Pick disease | 2.100 | 0.000 |
| pituitary cancer | -1.600 | 0.001 |
| PMID | Text |
|---|---|
| 24100239 | Study uncovers a first example of a vertebrate protein factor, Argonaute-3, specifically affecting the guide-to-passenger-strand ratio of the miRNA let-7a. A multi-layered mechanism for the observed impact of Ago3 on the let-7a-3p passenger strand expression and activity is proposed. |
| 23748378 | The Ago3 PIWI domain is slicing competent but our Ago3 model suggests multiple interactions between residues in the PIWI & N domains that may increase protein rigidity or invoke other properties that contribute to Ago3's observed slicing deficiency. |
| 23665583 | The N-terminal domain of Ago3 inhibits cleavage activity. AAs 1-64 are largely unstructured & form a large loop surrounding an area containing two helices & another unstructured loop. |
| 23064648 | DICER- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation. |
| 22474261 | Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression |
| 21634124 | reliable predictions of miRNA affinity to Ago2 AND Ago3 proteins were made. |
| 19393748 | EIF@C3 protein is expressed in both Schwann and neuron-type differentiating cells. |
| 18771919 | The specificity of RNA interference depends on the concentration of Ago1, Ago3, and Ago4 relative to Ago2. |
| 18187620 | Knockdown of eukaryotic translation initiation factor 2C, 3 (EIF2C3) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells |
MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSM 1 - 70 VQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVL 71 - 140 TGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPA 141 - 210 MWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKY 211 - 280 RVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAG 281 - 350 QRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQ 351 - 420 YGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQP 421 - 490 CFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQT 491 - 560 LSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATV 561 - 630 RVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQ 631 - 700 PGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLW 701 - 770 DDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDP 771 - 840 QALAKAVQIHQDTLRTMYFA 841 - 860 //
| PMID | Year | Title |
|---|---|---|
| 24100239 | 2013 | Argonaute-3 activates the let-7a passenger strand microRNA. |
| 23748378 | 2013 | Molecular dissection of human Argonaute proteins by DNA shuffling. |
| 23665583 | 2013 | Turning catalytically inactive human Argonaute proteins into active slicer enzymes. |
| 23186163 | 2013 | Toward a comprehensive characterization of a human cancer cell phosphoproteome. |
| 23064648 | 2012 | DICER- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation. |
| 22915799 | 2012 | HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies. |
| 22814378 | 2012 | N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. |
| 22795694 | 2012 | Slicing-independent RISC activation requires the argonaute PAZ domain. |
| 22681889 | 2012 | The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. |
| 22658674 | 2012 | Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. |
| More... |
