Tclin TOP1MT DNA topoisomerase I, mitochondrial

Protein Summary

Description

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Accession IDs

• Q969P6
• B7ZAR5
• E7ES89
• Q86ST4
• Q86V82
• TOP1mt

Gene Name

• TOP1MT

Ensembl ID

• ENST00000329245
• ENSP00000328835
• ENSG00000184428
• ENST00000519148
• ENSP00000429169
• ENST00000521193
• ENSP00000428369
• ENST00000523676
• ENSP00000429181

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
protein domain	1
histone modification site profile	0.92
transcription factor perturbation	0.8
virus perturbation	0.76
protein complex	0.72

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Dna Binding Protein / Dna Topoisomerase / Dna Topoisomerase I, Mitochondrial

DTO Classes

Protein / Enzyme / Isomerase / Dna Topoisomerase / Dna Topoisomerase I, Mitochondrial

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 25.94   (req: < 5)

Gene RIFs: 9   (req: <= 3)

Antibodies: 213   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 25.94   (req: >= 5)

Gene RIFs: 9   (req: > 3)

Antibodies: 213   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 4

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 1