| Tbio | U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase |
RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753). In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).
Comments
| Disease | Target Count | P-value |
|---|---|---|
| ependymoma | 4679 | 2.3e-07 |
| atypical teratoid / rhabdoid tumor | 5112 | 7.9e-06 |
| medulloblastoma, large-cell | 6241 | 4.4e-05 |
| ovarian cancer | 8520 | 9.0e-05 |
| psoriasis | 6694 | 7.6e-04 |
| primitive neuroectodermal tumor | 3035 | 1.8e-03 |
| group 3 medulloblastoma | 4104 | 4.5e-03 |
| osteosarcoma | 7950 | 6.4e-03 |
| Disease | log2 FC | p |
|---|---|---|
| atypical teratoid / rhabdoid tumor | 1.200 | 7.9e-06 |
| ependymoma | 1.100 | 2.3e-07 |
| group 3 medulloblastoma | 1.200 | 4.5e-03 |
| medulloblastoma, large-cell | 1.400 | 4.4e-05 |
| osteosarcoma | 1.103 | 6.4e-03 |
| ovarian cancer | -1.300 | 9.0e-05 |
| primitive neuroectodermal tumor | 1.200 | 1.8e-03 |
| psoriasis | -1.100 | 7.6e-04 |
MALSKSMHARNRYKDKPPDFAYLASKYPDFKQHVQINLNGRVSLNFKDPEAVRALTCTLLREDFGLSIDI 1 - 70 PLERLIPTVPLRLNYIHWVEDLIGHQDSDKSTLRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFN 71 - 140 YAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEESEIIYDFCMCNPPFFANQLEAKGVNSRNPRRPPPSSV 141 - 210 NTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTYTEFCQGRT 211 - 280 MRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLASVMKELSLKASPLRSETAEGIVVVTTWIEKIL 281 - 350 TDLKVQHKRVPCGKEEVSLFLTAIENSWIHLRRKKRERVRQLREVPRAPEDVIQALEEKKPTPKESGNSQ 351 - 420 ELARGPQERTPCGPALREGEAAAVEGPCPSQESLSQEENPEPTEDERSEEKGGVEVLESCQGSSNGAQDQ 421 - 490 EASEQFGSPVAERGKRLPGVAGQYLFKCLINVKKEVDDALVEMHWVEGQNRDLMNQLCTYIRNQIFRLVA 491 - 560 VN 561 - 562 //
