| Tbio | Ubiquitin-conjugating enzyme E2 B |
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
Comments
| Disease | Target Count | P-value |
|---|---|---|
| ependymoma | 2514 | 1.18705839488156E-8 |
| ovarian cancer | 8492 | 8.43298274699273E-8 |
| lung adenocarcinoma | 2714 | 2.85399779371425E-7 |
| atypical teratoid / rhabdoid tumor | 4369 | 6.83659422238274E-7 |
| limb girdle muscular dystrophy 2B | 74 | 1.33966310052178E-6 |
| tuberculosis and treatment for 3 months | 327 | 1.98122006561003E-6 |
| lung cancer | 4473 | 3.44632917795864E-5 |
| medulloblastoma, large-cell | 6234 | 3.61224638336254E-5 |
| psoriasis | 6685 | 2.87790350176395E-4 |
| glioblastoma | 5572 | 3.26731756834004E-4 |
| primitive neuroectodermal tumor | 3031 | 5.21994625106996E-4 |
| adult high grade glioma | 2148 | 0.00267086564938272 |
| Multiple myeloma | 1328 | 0.00280518969851762 |
| astrocytic glioma | 2241 | 0.0151172841726721 |
| oligodendroglioma | 2849 | 0.0325278718854453 |
| spina bifida | 1064 | 0.0456940366255198 |
| Disease | Target Count | Z-score | Confidence |
|---|---|---|---|
| Male infertility | 170 | 4.264 | 2.1 |
| Aggressive systemic mastocytosis | 6 | 3.099 | 1.5 |
| Disease | log2 FC | p |
|---|---|---|
| Multiple myeloma | 1.262 | 0.003 |
| astrocytic glioma | -1.700 | 0.015 |
| oligodendroglioma | -1.400 | 0.033 |
| psoriasis | 1.200 | 0.000 |
| ependymoma | -1.200 | 0.000 |
| atypical teratoid / rhabdoid tumor | -1.900 | 0.000 |
| glioblastoma | -1.400 | 0.000 |
| medulloblastoma, large-cell | -1.900 | 0.000 |
| primitive neuroectodermal tumor | -1.100 | 0.001 |
| limb girdle muscular dystrophy 2B | -1.156 | 0.000 |
| tuberculosis and treatment for 3 months | -1.100 | 0.000 |
| lung cancer | -1.900 | 0.000 |
| adult high grade glioma | -1.300 | 0.003 |
| lung adenocarcinoma | -1.100 | 0.000 |
| spina bifida | -1.004 | 0.046 |
| ovarian cancer | -2.300 | 0.000 |
| Species | Source |
|---|---|
| Chimp | OMA EggNOG |
| Macaque | OMA EggNOG Inparanoid |
| Mouse | OMA EggNOG Inparanoid |
| Cow | OMA Inparanoid |
| Anole lizard | OMA Inparanoid |
| Xenopus | OMA Inparanoid |
| C. elegans | OMA Inparanoid |
| S.cerevisiae | OMA Inparanoid |
| PMID | Text |
|---|---|
| 26679603 | These findings show the importance of Rad6 in ovarian cancer and emphasize the need for further studies of Rad6 as a potential target for the treatment of ovarian cancer. |
| 26336826 | Data show that the ubiquitin-conjugating enzyme E2 RAD6A/B-MDM2 ubiquitin ligase machinery regulates anti-silencing function 1A protein (ASF1A) degradation. |
| 26223869 | A novel variant (Chr5.133706925 A > G) residing in the UBE2B gene promoter region confers a high risk for idiopathic azoospermia in a Chinese population. |
| 25384975 | RAD6 physically interacts with heterochromatin protein 1alpha and ubiquitinates HP1alpha at residue K154, thereby promoting heterochromatin protein 1alpha degradation through the autophagy pathway |
| 23525009 | RNF168, in complex with RAD6A or RAD6B, is activated in the DNA-damage-induced protein ubiquitination cascade. |
| 23378580 | UBE2B mRNA alterations are associated with severe oligozoospermia in infertile men. |
| 23079972 | UBE2B polymorphisms g.-293T>G, g.20016A>G and g.9157A>G are not associated with male infertility, and the GA haplotype is likely a protective factor for male fertility in Northeast Chinese men. |
| 22705350 | beta-catenin and Rad6B interacting regions, were identified. |
| 22547805 | The Rad6-Rad18 enzyme plays an essential role in promoting replication through DNA lesions by translesion synthesis in mammalian cells. |
| 22083959 | RAD6 can form a ternary complex with MDM2 and p53 that contributes to the degradation of p53. |
| More... |
| PMID | Year | Title |
|---|---|---|
| 26679603 | 2016 | Rad6 upregulation promotes stem cell-like characteristics and platinum resistance in ovarian cancer. |
| 26336826 | 2015 | A conserved RAD6-MDM2 ubiquitin ligase machinery targets histone chaperone ASF1A in tumorigenesis. |
| 26223869 | 2015 | A functional variant in the UBE2B gene promoter is associated with idiopathic azoospermia. |
| 25384975 | 2015 | RAD6 promotes homologous recombination repair by activating the autophagy-mediated degradation of heterochromatin protein HP1. |
| 23525009 | 2013 | RNF168 forms a functional complex with RAD6 during the DNA damage response. |
| 23378580 | 2013 | UBE2B mRNA alterations are associated with severe oligozoospermia in infertile men. |
| 23079972 | 2012 | Genetic association of UBE2B variants with susceptibility to male infertility in a Northeast Chinese population. |
| 22705350 | 2012 | Lysine 394 is a novel Rad6B-induced ubiquitination site on beta-catenin. |
| 22547805 | 2012 | Requirement of Rad18 protein for replication through DNA lesions in mouse and human cells. |
| 22083959 | 2012 | RAD6 regulates the dosage of p53 by a combination of transcriptional and posttranscriptional mechanisms. |
| More... |
