Tchem NEK2 Serine/threonine-protein kinase Nek2

Protein Summary

Description

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856). Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells. This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Accession IDs

• P51955
• Q53FD6
• Q5I1Z9
• Q5VXZ1
• Q6NZX8
• Q7Z634
• Q86XH2
• Q96QN9

Gene Name

• NEK2

Ensembl ID

• ENST00000366998
• ENSP00000355965
• ENSG00000117650
• ENST00000366999
• ENSP00000355966

Symbol

• NEK2A
• NLK1
• NLK1
• RP67
• NEK2A
• HsPK21
• PPP1R111

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
molecular function	0.96
histone modification site profile	0.89
drug perturbation	0.87
transcription factor binding site profile	0.84
PubMedID	0.81

Protein Classes

PANTHER Classes

Protein / Transferase / Kinase / Protein Kinase / Serine/threonine-protein Kinase Nek2

DTO Classes

Protein / Kinase / Protein Kinase / Other Group / Nek Family / Serine/threonine-protein Kinase Nek2

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 224.99   (req: < 5)

Gene RIFs: 122   (req: <= 3)

Antibodies: 423   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 224.99   (req: >= 5)

Gene RIFs: 122   (req: > 3)

Antibodies: 423   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 27

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligands: 17

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0