Tbio TOP3A DNA topoisomerase 3-alpha

Protein Summary

Description

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity (PubMed:30057030). It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2 (PubMed:29290614). This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Accession IDs

• Q13472
• A8KA61
• B4DK80
• D3DXC7
• Q13473

Gene Name

• TOP3A

Ensembl ID

• ENST00000321105
• ENSP00000321636
• ENSG00000177302
• ENST00000542570
• ENSP00000442336
• ENST00000580095
• ENSP00000462790
• ENST00000638894
• ENSP00000492229
• ENSG00000284238
• ENST00000639065
• ENSP00000491260
• ENST00000640743
• ENSP00000491689

Symbol

• TOP3
• TOP3
• PEOB5
• ZGRF7
• MGRISCE2

Knowledge Table

Most Knowledge About	Knowledge Value (0 to 1 scale)
protein domain	1
transcription factor binding site profile	0.89
tissue	0.72
cellular component	0.7
histone modification site profile	0.69

Protein Classes

PANTHER Classes

Protein / Nucleic Acid Binding / Dna Topoisomerase 3-alpha

Protein / Isomerase / Dna Topoisomerase 3-alpha

Protein / Enzyme Modulator / Dna Topoisomerase 3-alpha

DTO Classes

Protein / Enzyme / Isomerase / Dna Topoisomerase 3-alpha

IDG Development Level Summary

Tdark

These are targets about which virtually nothing is known. They do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 250.49   (req: < 5)

Gene RIFs: 26   (req: <= 3)

Antibodies: 69   (req: <= 50)

Tbio

These targets do not have known drug or small molecule activities
- AND - satisfy two or more of the following criteria:

Pubmed score: 250.49   (req: >= 5)

Gene RIFs: 26   (req: > 3)

Antibodies: 69   (req: > 50)

- OR - satisfy the following criterion:

Gene Ontology Terms: 10

Tchem

Target has at least one ChEMBL compound with an activity cutoff of < 30 nM - AND - satisfies the preceding conditions

Active Ligand: 0

Tclin

Target has at least one approved drug - AND - satisfies the preceding conditions

Active Drug: 0