Property Summary

NCBI Gene PubMed Count 33
PubMed Score 73.45
PubTator Score 43.73

Knowledge Summary


No data available


  Differential Expression (22)

Disease log2 FC p
Multiple myeloma 1.641 2.0e-03
malignant mesothelioma 2.200 2.6e-07
esophageal adenocarcinoma 1.100 2.1e-02
astrocytoma 1.100 4.3e-09
glioblastoma 1.800 1.2e-07
oligodendroglioma 1.200 1.3e-08
sonic hedgehog group medulloblastoma 2.400 4.0e-07
medulloblastoma, large-cell 1.800 7.9e-06
primitive neuroectodermal tumor 1.800 7.0e-04
adrenocortical carcinoma 1.813 6.9e-05
non-small cell lung cancer 1.677 1.0e-20
intraductal papillary-mucinous carcinoma... 1.500 2.4e-03
intraductal papillary-mucinous neoplasm ... 1.400 6.4e-03
lung cancer 2.000 1.3e-02
colon cancer 1.200 9.6e-03
pediatric high grade glioma 1.800 5.8e-06
primary Sjogren syndrome 1.100 4.6e-03
nasopharyngeal carcinoma 1.300 3.5e-05
ductal carcinoma in situ 1.700 4.8e-04
invasive ductal carcinoma 2.000 1.8e-04
ovarian cancer 1.500 2.6e-04
psoriasis 1.200 1.1e-45

 GO Component (1)

 MGI Phenotype (1)

Gene RIF (18)

24287290 The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells.
22123827 Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
21536671 bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase.
21036905 Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction
19864417 The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK.
19744312 Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle.
19111665 These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling.
18714392 increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies
18536724 A strong origin of replication at the DBF4 promoter locus, which contains two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions, is identified.
18503552 ASK/Dbf4, a novel cell survival gene in melanoma is transcriptionally regulated by E2F1.
18286467 Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells
17768177 There is the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggest that up-regulation of ASK/Dbf4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in cutaneous melanoma.
17276990 Overexpression of Dbf4 abrogates the S checkpoint response to ultraviolet radiation but not ionizing radiation.
16899510 Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells.
16826239 Cdc7-Dbf4 kinase efficiently phosphorylates p150.
12535533 Results demonstrate a DNA damage checkpoint that targets Cdc7/Dbf4 protein kinase.
12420215 The HuDbf4 gene has of 12 exons over a 33-kb region. The MluI cell-cycle box is needed for core promoter activation. The Sp1 upregulator & HES-1 repressor coordinately determine promoter efficiency. Transcription initiations occur at -220, -235 and -245.
12015319 results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site

AA Sequence


Text Mined References (45)

PMID Year Title
24287290 2014 The cell cycle arrest and the anti-invasive effects of nitrogen-containing bisphosphonates are not mediated by DBF4 in breast cancer cells.
23186163 2013 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
22123827 2012 Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
21536671 2011 Molecular mechanism of activation of human Cdc7 kinase: bipartite interaction with Dbf4/activator of S phase kinase (ASK) activation subunit stimulates ATP binding and substrate recognition.
21406692 2011 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
21036905 2010 Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction.
20068231 2010 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
19864417 2010 Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity.
19744312 2009 Identification of secondary targets of N-containing bisphosphonates in mammalian cells via parallel competition analysis of the barcoded yeast deletion collection.
19690332 2009 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
19413330 2009 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19369195 2009 Large-scale proteomics analysis of the human kinome.
19111665 2008 The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control.
18714392 2008 Cdc7-Dbf4 kinase overexpression in multiple cancers and tumor cell lines is correlated with p53 inactivation.
18691976 2008 Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.
18669648 2008 A quantitative atlas of mitotic phosphorylation.
18536724 2008 Asymmetric bidirectional replication at the human DBF4 origin.
18503552 2008 Transcriptional regulation of ASK/Dbf4 in cutaneous melanoma is dependent on E2F1.
18286467 2008 Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells.
17768177 2007 Identification and functional characterization of ASK/Dbf4, a novel cell survival gene in cutaneous melanoma with prognostic relevance.
17525332 2007 ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
17276990 2007 Cdc7-Dbf4 and the human S checkpoint response to UVC.
17062569 2007 Cdc7 is an active kinase in human cancer cells undergoing replication stress.
16964243 2006 A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
16899510 2006 Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.
16826239 2006 The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigen.
15707391 2005 DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system.
15489334 2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15374998 2004 Functional interaction between tumor suppressor menin and activator of S-phase kinase.
15302935 2004 Large-scale characterization of HeLa cell nuclear phosphoproteins.
15231747 2004 A protein interaction framework for human mRNA degradation.
15226314 2004 Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures.
14702039 2004 Complete sequencing and characterization of 21,243 full-length human cDNAs.
12853948 2003 The DNA sequence of human chromosome 7.
12791985 2003 Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes.
12690205 2003 Human chromosome 7: DNA sequence and biology.
12614612 2003 Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.
12535533 2003 An ATR- and Cdc7-dependent DNA damage checkpoint that inhibits initiation of DNA replication.
12477932 2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
12420215 2002 Human Dbf4/ASK promoter is activated through the Sp1 and MluI cell-cycle box (MCB) transcription elements.
12015319 2002 A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase.
10846177 2000 Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 bY Cdks.
10523313 1999 Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication.
10517317 1999 Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes.
10373557 1999 A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells.